Nanodisc based Membrane Protein Incorporation Service

The nanodiscs is a non-covalent structure of lipid bilayer and membrane scaffold protein (MSP). MSPs are genetically engineered proteins, which derived from human apolipoprotein A-1 to shield the hydrophobic core of the lipids from the aqueous phase. The size of the nanodiscs was controlled by the length of the MSPs. The diameter of nanodiscs is typically in the order of ~10 nm, but the generation of specific MSP variants allows the formation of smaller (6-7 nm) and larger (16-17 nm) nanodiscs. Lipid-bilayer nanodiscs are the most powerful tool in solubilizing/incorporating membrane proteins in a cell membrane-like environment. This solubilization/incorporation system represents a very suitable option if you need to keep the folded conformation, intact function, and oligomerization of your target proteins all in their native forms.

Membrane Protein Incorporation in Nanodiscs

In nanodiscs assembly, we will first solubilize your membrane proteins using detergents and purify them in a micellar solution. Since different detergents and detergent mixtures may affect the stability, structure, function, and activity of a membrane protein very differently. We can also use our detergent screening platform to find the most suitable detergent or detergent mixture for your membrane proteins. After the solubilization and purification, we will add lipids and the membrane scaffolding protein (MSP) to the micellar solution of membrane proteins followed by removing the detergents via hydrophobic beads binding or dialysis.

Membrane Protein Incorporation in Nanodiscs (Creative Biolabs)

Advantages of Membrane Protein Incorporation in Nanodiscs

Best mimic the native environment of membrane proteins
Highly homogeneous with the controllable size and membrane protein/lipid ratio
Stable form
Detergent-free
Stabilize membrane proteins in either the monomeric or designated oligomeric form by fixing the diameter of the nanodiscs
Have a wide range of applications

Applications of Membrane Protein Incorporation in Nanodiscs

Nanodiscs are soluble nanoscale phospholipid bilayers that can self-assemble integral membrane proteins for biophysical, enzymatic, or structural investigations.

Antibody Discovery
Fluorescence Spectroscopy
Solution-state NMR and Solid-state NMR
Mass Spectrometry
Other Biochemical Techniques, such as Cryo-EM, single-molecule characterizations, surface plasmon resonance, biolayer, interferometry (BLI), micro-ring resonators, charge-sensitive optical detection, scintillation proximity assay

Creative Biolabs has been dedicated to providing first-class membrane protein production service using a variety of strategies. Based on our leading-edge platform, we have successfully produced, purified, stabilized, and characterized many challenging membrane protein targets. Nanodiscs are almost the perfect tool for solubilizing, reconstituting, and incorporating membrane proteins in a cell membrane-like environment. At Creative Biolabs, we guarantee our clients the most qualified and tailored service to satisfy each specific demand. Besides, you can also choose our services in solubilization/reconstitution of membrane proteins in liposomes, micelles, and amphipols. Please feel free to inquire us for more information.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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