Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsLeveraging our cutting-edge platforms, Creative Biolabs specializes in the discovery of single domain antibodies (sdAbs) with high affinity and specificity. Based on our clients' requirements, we could immunize camels, llamas, alpacas, or even sharks as repertoire source to develop single domain antibodies targeting various antigens.
Alternatively, our team can screen our Premade Single Domain Antibody (SdAb) Libraries to isolate high-potency single domain antibodies against a large number of challenge targets. In addition to our sdAb discovery services, we offer a comprehensive range of Recombinant Single Domain Antibody (SdAb) Products to meet all your needs.
Expert guidance in project design, target selection, and strategy development to meet specific project requirements.
~2 weeks
Design and production of antigens tailored to a wide range of targets such as large protein molecules, membrane proteins, haptens, or other challenging targets, ensuring optimal antibody generation.
~6 weeks
Comprehensive options are available at Creative Biolabs to initiate your sdAb discovery projects.
High-throughput library screening & validation will be conducted with the most suitable strategy to select candidate sdAbs with desired properties, such as specificity, affinity, stability, or functionality.
1-4 months
Various characterization assays of sdAbs upon your request to ensure suitability for intended properties:
Through tailored strategies to optimize sdAbs with desired properties for specific applications (e.g., therapeutics, diagnostics, or research), which including but not limited to:
Various expression systems, such as bacteria, yeast, or mammalian cells, are set up by Creative Biolabs to express and purify sdAbs with high purity and activity. Meanwhile, our scalable production system offers flexible solutions to meet your specific demands on scaling up sdAb manufacture.
6 weeks
Phage display has emerged as a powerful tool for the discovery and optimization of sdAbs. This in vitro selection technique leverages bacteriophages - viruses that infect bacteria - to present sdAbs on their surface. By linking the displayed sdAb, phage display enables the rapid identification of high-affinity sdAbs from vast libraries of variants.
Yeast display allows efficient screening and selection of high-affinity sdAb binders against cell line targets. The eukaryotic nature of yeast enables proper folding and post-translational modifications of the displayed binders, which is crucial for maintaining the functionality of sdAbs.
B cell repertoire is a potential source of sdAbs. Single B cell sorting technology has advanced significantly recently, enhancing efficiency and feasibility in rapidly obtaining specific sdAbs. Our cutting-edge Native™ platform encompasses B cell screening, cloning, and in vitro expression, and offers an innovative solution for discovering and developing novel sdAbs.
Llama Immunization with Good Response
Constructed SdAb Library with High Accuracy
Positive SdAb Binders Enrichment
High-Quality SdAb Production
Ready to harness the power of single domain antibodies for your research or therapeutic development? today to discuss your project requirements and explore how our services can accelerate your success.
A: Single domain antibody (sdAb), also known as a domain antibody, VHH, or VNAR, is a kind of antibody fragment consisting of a single monomeric variable antibody domain. It lacks the light chain and the CH domain of the heavy chain found in the conventional Fab region. With a molecular weight of only 12-15 kDa, it is significantly smaller than a full-length antibody (150-160 kDa) or other antibody fragments such as Fab (~50 kDa) and scFv (~25 kDa).
Single domain antibodies represent a powerful class of molecules with unique properties that make them valuable tools for biomedical research and therapeutic development. Their versatility, stability, and specificity have led to their widespread adoption in numerous applications, driving innovation in various fields of science and medicine.
A: sdAbs have several advantages, including small size, high stability, and solubility, and the ability to target unique epitopes with high specificity. They can target epitopes inaccessible to conventional antibodies and have the potential for oral delivery and tissue penetration. Additionally, sdAbs can be easily engineered for various applications.
A: Single domain antibodies can be generated with immunization of camelids (e.g., llamas, camels) or directly screen sdAbs with desired properties from a synthetic library. These methods involve isolating sdAbs with high affinity and specificity for a target antigen from diverse antibody repertoires coupled with display technologies such as phage display or yeast display.
A: Single domain antibodies have diverse applications across research, diagnostics, therapeutics, and biotechnology. They can be used for target validation, imaging, drug delivery, protein purification, and also as research reagents. Their small size and unique properties make them valuable tools in various fields.
A: Although single domain antibodies have many advantages in drug development, their production and development processes also face some challenges. The first is to ensure the high affinity and specificity of sdAbs, which is very important for their effectiveness. Therefore, through fine engineering design and screening, it is necessary to develop single domain antibodies with high affinity and high specificity. In addition, single domain antibodies usually have good tissue permeability and stability. However, in practical applications, the half-life of sdAbs is short, which limits their effectiveness in some therapeutic applications. To overcome this problem, researchers are developing strategies such as binding to long-acting serum albumin or prolonging its half-life in the body by changing its molecular structure. Finally, how to achieve high yield expression and purification of single domain antibodies is still a technical challenge. This requires meticulous process development and optimization to ensure that the produced single domain antibodies have the required purity, activity, and safety.
A: The common method is to use phage display technology or other surface display technology. Through these techniques, single domain antibodies with high affinity and specificity for specific antigens can be screened from a large antibody library. In addition, directed evolution is another strategy to enhance the affinity of sdAbs. By mutating the key amino acids of sdAbs, a large number of variants are generated, from which the variants with higher affinity for specific antigens can be screened. This process can be repeated to gradually improve the affinity of single domain antibodies. The antibody can also be humanized by molecular engineering technology to reduce immunogenicity while maintaining its affinity and specificity. This is particularly important for the development of single domain antibodies for clinical treatment.
A: Our services encompass the entire process of single domain antibody development, from antigen selection, animal immunization, library construction, to screening, characterization, affinity maturation, and scale-up production. We also offer customized services tailored to specific project needs.
A: We primarily utilize llamas, alpacas, camels, or synthetic libraries for sdAb discovery. However, we are flexible and can discuss alternative options based on project requirements and client preferences.
A: The timeline varies depending on the specific project requirements and factors such as the complexity of the target, the desired affinity, and the selected method of generation. Typically, the process can range from a few months for discovery to additional time for optimization and production. We work closely with clients to establish timelines that meet their needs.
A: We specialize in generating sdAbs against challenging targets, including small molecules, peptides, membrane proteins, and highly conserved epitopes. Our team has extensive experience in target validation and optimization to tackle challenging antigens.
A: To start a project or inquire further about our single domain antibody services, please contact our team, through online inquiry box, email info@creative-biolabs.com, or call +1-631-416-1478 directly, with details about your requirements, including the target antigen, intended applications, timeline, and any specific customization needs. We look forward to collaborating with you to achieve your goals.
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All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
