"Creative Biolabs is committed to providing highly customized comprehensive solutions with the best quality to advance our global clients’ projects."
Manufacturing Cell Line Development
Cell Line Construction:
The basis of manufacturing biologically active products relies on generating high quality cell lines which comply with current regulatory guidelines. However, the construction of a validated cell line requires sophisticated and time-consuming work. Scientists from Creative Biolabs use Glutamine Synthetase (GS) or Dihydrofolate Reductase (DHFR) systems to construct the stable cell lines. Recombinant clones will be selected for target expression analysis and qualified by their performance in a functional cell-based assay. We develop not only high expressing clones but also those which are capable of a successful transfer to large scale protein production.
Our Services Include:
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Cloning of the provided cDNA into a suitable expression vector. Codon optimizations can be introduced for the high yield production.
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Transfection of host cell line which is in compliance with FDA guideline.
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Selection of stably transfected cells.
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Isolation of cell clones by efficient and time-saving strategy.
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Screening of clones by target expression titration.
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Selection of recombinant cell lines at the highest expression level and preparation of cryostocks..
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Quality control of cell line stocks for viability and sterility including mycoplasma testing.
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Free cell line backup service for two years from delivery of cell clones.
Cell Banking Services:
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Independent and dedicated suites for establishing cGMP cell bank.
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Cells grown, maintained and stored within validated incubators and freezers (liquid nitrogen).
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24-hour security monitoring and comprehensive environmental monitoring.
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Single-pass, positive-pressure, HEPA-filtered air unidirectional flow of personnel and materials with appropriate air locks.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.