Rabbit Reticulocyte Lysate based Cell-free Membrane Protein Expression Service

Cell-free Rabbit Reticulocyte Lysate (RRL) used for membrane protein expression is generated from lysed reticulocytes isolated from rabbits treated with phenylhydrazine. The lysed reticulocytes are pre-treated with micrococcal nuclease to remove endogenous mRNA. This system is now widely employed to elucidate the mechanisms of mammalian post-translational modifications, translation, co-translational modifications, and translocation of proteins. Particularly, it can also be incorporated in microarray, display, or in vitro expression cloning (IVEC) technologies for protein evolution, interaction, selection studies. Taken together, rabbit reticulocyte lysate system makes an attractive tool for structural biology, functional genomics, drug screening, and vaccine production.

Overview

Be able to efficiently translate various high molecular proteins from either capped or uncapped mRNA.
Facilitate the production of membrane proteins at high levels.
Simple protocol and easy setup with all necessary components.
Membrane protein of interest can be synthesized and visualized in a few hours.
Available to large volume of template mRNA.

Kit Description

Magic™ Cell-free Membrane Protein Expression in Rabbit Reticulocyte kit is a cell-free membrane protein synthesis system using rabbit retic lysate and is optimized for a variety of high specific activity protein synthesis from capped and uncapped mRNA and appropriated protein folding. The kit contains the required components for protein synthesis. The optimized Reticulocyte Lysate in kit provides ATP-regeneration system, hemin, and calf liver tRNA. The optimized Translation Mix (-Met or -Leu) for the translation of both capped and uncapped messages along with High and Low Salt buffers. Which allows protein synthesis to occur at high efficiency, protein yield, and biological activity.

Components List and Storage

Expression System	Components	Storage
In Vitro Rabbit Reticulocyte Expression System	Retic Lysate	-80°C
	20X Translation Mix minus-Met	-80°C
	20X Translation Mix minus-Leu	-80°C
	High Salt Mix minus-Met	-80°C
	Low Salt Mix minus-Met	-80°C
	Capped Xen-1α RNA (50 μg/mL)	-80°C
	Nuclease-Free Water	Any temp

Advantages

Compared with conventional cell-based/cell-free protein synthesis platforms, RRL system exerts multiple superior features
Easily prepared
Cap-independent translation
Compatible with mammalian post-translational protein modification system
Large proteins greater than 30 kDa can be expressed efficiently
Compatible with high-throughput format for protein microarrays and other screening procedures

Applications

In terms of the RRL expression kit, clients can use it to express the proteins of interest. For instance, it can efficiently express in vitro-synthesized transcripts, poly(A) RNA, and total RNA, including difficult-to-translate RNAs. It is noticed that this expression system can produce a large amount of high specific activity protein from the target gene when radiolabeled amino acid is added into the reaction. Subsequently, users can analyze the synthesized protein by several methods, such as SDS-PAGE, TCA precipitation, WB, and functional analysis.

Creative Biolabs is a long-term expert in the field of protein production. After years of dedication, we have successfully built and optimized a versatile cell-free membrane protein synthesis platform which is proved to be very effective and powerful for many difficult protein targets. Our scientists are experienced in tailoring the most reliable and cost-effective plans for our global clients to facilitate their diverse research programs. Equipped with state-of-the-art technology platforms and extensive expertise, our one-stop integrated Magic™ protein production service in cell-free rabbit reticulocyte lysate expression system can help our clients in the world to boost their diverse research and projects. To discuss your plans for membrane protein expression, please feel free to contact us for more details.

Related Products

Cat	Product Name	Expected Yield of Positive Control
MPCFK-6	Magic™ Cell-free Membrane Protein Expression in Rabbit Reticulocyte Lysate	Based on RNA purity

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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