How does the cleavable linker in the anti-MIgG(Fc)Fab-C-DMDM ADC function?

The cleavable linker in the anti-MIgG(Fc)Fab-C-DMDM ADC is designed to release the cytotoxic drug, DMDM, upon internalization into the target cell. This allows for precise delivery of the drug to the intended site, minimizing potential damage to non-target cells and enhancing the therapeutic efficacy of the ADC.

Can the anti-MIgG(Fc)Fab-C-DMDM ADC be used for in vivo studies?

Yes, the anti-MIgG(Fc)Fab-C-DMDM ADC is suitable for in vivo studies, especially for preclinical models using mouse monoclonal antibodies. Its design allows researchers to assess the therapeutic potential of new ADCs efficiently and economically.

What types of cancer cells can be targeted with the anti-MIgG(Fc)Fab-C-DMDM ADC?

The targeting scope of the anti-MIgG(Fc)Fab-C-DMDM ADC depends on the primary antibody to which it is conjugated. It is primarily used for screening purposes in preclinical settings to identify potential ADC candidates that can specifically bind to antigens expressed on certain cancer cells.

What experimental applications are most suitable for the anti-MIgG(Fc)Fab-C-DMDM ADC?

The anti-MIgG(Fc)Fab-C-DMDM ADC is primarily designed for use in ADC screening experiments and in vitro cytotoxicity assays. It is particularly effective in pre-screening mouse monoclonal antibodies to identify potential ADC candidates that target specific tumor antigens.

How should experiments be designed to test the efficacy of the anti-MIgG(Fc)Fab-C-DMDM ADC?

Experiments testing the efficacy of the anti-MIgG(Fc)Fab-C-DMDM ADC should include control groups using non-targeted ADCs, dose-response studies to determine optimal concentrations, and time-course studies to observe the duration of cytotoxic effects. It is also beneficial to include assays that measure direct ADC binding to target cells and subsequent immune cell recruitment.

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anti-MIgG(Fc)Fab-C-DMDM ADC

anti-MIgG(Fc)Fab-C-DMDM ADC (CAT#: ADC-AA-030)

This ADC product is comprised of a Fab fragment of an anti-mouse IgG Fc specific polyclonal antibody conjugated via a cleavable linker to DMDM. The antibody portion is a Fab fragment of a secondary antibody and the drug portion, DMDM, is a cytotoxic small molecule which binds to DNA, causes DNA damage. This product displays no obvious toxicity without a primary antibody and can be a quite efficient and economical alternative to pre-screening mouse monoclonal antibodies as ADC candidates.

ADC Target
ADC Antibody
ADC Linker
ADC payload drug

Name
IgG Fc

Overview
The fragment crystallizable region (Fc region) is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc region of IgG bears a highly conserved N-glycosylation site. Glycosylation of the Fc fragment is essential for Fc receptor-mediated activity. Fc binds to various cell receptors and complement proteins thus mediating different physiological effects of antibodies, such as opsonization, antibody dependent cellular cytotoxicity (ADCC), degranulation of mast cells, basophils, eosinophils and other processes.

Overview
Fab fragment of anti-mouse IgG Fc specific polyclonal antibody

Species Reactivity
Mouse

Name
Cleavable linkers

Description
Cleavable linkers rely on the physiological stimuli, which mainly include chemically cleavable linkers and enzymatically cleavable linkers. Chemically cleavable linkers including acid-labile linkers and disulfide linkers. For acid-labile linkers, intracellular release of payloads relies on the different pH between endosomes/lysosomes and blood. The release of disulﬁde-linked drugs is controlled by the factors in intracellular environment. Enzymatically cleavable linkers, peptide linkers and β-glucuronide linkers, are sensitive to enzymes located in cytoplasm.

Name
DMDM (duocarmycin DM)

Description
Duocarmycins belong to the minor-groove-binding DNA-alkylating agents (DNA MGBA). The natural duocarmycins are isolated from the culture broth of Streptmyces sp and a series of derivatives has also been synthesized.

For Research Use Only. NOT FOR CLINICAL USE.

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Customer Reviews FAQ

Excellent

Creative Biolabs' anti-MIgG(Fc)Fab-C-DMDM ADC exceeded expectations in our labs. Its ability to target and damage DNA without off-target effects helped advance our antibody research significantly.

Excellent

In our quest for efficient ADC candidates, the anti-MIgG(Fc)Fab-C-DMDM has been instrumental. It offers precise targeting with minimal toxicity, streamlining our processes.

Excellent

The efficiency and safety profile of the anti-MIgG(Fc)Fab-C-DMDM ADC make it indispensable for our lab's antibody screening protocols.

Excellent

The anti-MIgG(Fc)Fab-C-DMDM ADC from Creative Biolabs offers precise DNA damage, enhancing our antibody candidate selection with its non-toxic and efficient profile.

Excellent

Our lab has successfully integrated this ADC into our workflows, seeing substantial improvements in screening efficiency and accuracy.

Q: 1: How does the cleavable linker in the anti-MIgG(Fc)Fab-C-DMDM ADC function?
A: The cleavable linker in the anti-MIgG(Fc)Fab-C-DMDM ADC is designed to release the cytotoxic drug, DMDM, upon internalization into the target cell. This allows for precise delivery of the drug to the intended site, minimizing potential damage to non-target cells and enhancing the therapeutic efficacy of the ADC.
Q: 2: Can the anti-MIgG(Fc)Fab-C-DMDM ADC be used for in vivo studies?
A: Yes, the anti-MIgG(Fc)Fab-C-DMDM ADC is suitable for in vivo studies, especially for preclinical models using mouse monoclonal antibodies. Its design allows researchers to assess the therapeutic potential of new ADCs efficiently and economically.
Q: 3: What types of cancer cells can be targeted with the anti-MIgG(Fc)Fab-C-DMDM ADC?
A: The targeting scope of the anti-MIgG(Fc)Fab-C-DMDM ADC depends on the primary antibody to which it is conjugated. It is primarily used for screening purposes in preclinical settings to identify potential ADC candidates that can specifically bind to antigens expressed on certain cancer cells.
Q: 4: What experimental applications are most suitable for the anti-MIgG(Fc)Fab-C-DMDM ADC?
A: The anti-MIgG(Fc)Fab-C-DMDM ADC is primarily designed for use in ADC screening experiments and in vitro cytotoxicity assays. It is particularly effective in pre-screening mouse monoclonal antibodies to identify potential ADC candidates that target specific tumor antigens.
Q: 5: How should experiments be designed to test the efficacy of the anti-MIgG(Fc)Fab-C-DMDM ADC?
A: Experiments testing the efficacy of the anti-MIgG(Fc)Fab-C-DMDM ADC should include control groups using non-targeted ADCs, dose-response studies to determine optimal concentrations, and time-course studies to observe the duration of cytotoxic effects. It is also beneficial to include assays that measure direct ADC binding to target cells and subsequent immune cell recruitment.

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CAT#	Product Name	Linker	Payload
ADC-AA-029	anti-MIgG(Fc)Fab-C-MMAE ADC	Cleavable linkers	MMAE (Monomethyl auristatin E)
ADC-AA-027	anti-MIgG(Fc)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-048	Protein G-VC-MMAE ADC	VC (valine-citrulline)	MMAE (Monomethyl auristatin E)
ADC-AA-049	Protein G-MMAF ADC		MMAF (Monomethyl auristatin F)
ADC-AA-054	Protein A-MCC-DM1 ADC	MCC (Maleimidomethyl cyclohexane-1-carboxylate)	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)

CAT#	Product Name	Linker	Payload
ADC-AA-021	anti-MIgG(Fc)-C-MMAE ADC	Cleavable linkers	MMAE (Monomethyl auristatin E)
ADC-AA-066	Anti-Rat IgG(H+L)-C-PBD ADC	Cleavable linkers	PBD (pyrrolobenzodiazepine)
ADC-AA-010	anti-HIgG(Fc)Fab-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
WJY-0423-LS106	Protein A-Calicheamicin ADC	Cleavable linkers	Calicheamicin
ADC-AA-062	Anti-HIgG(Fc)Fab-C-DX8951 ADC	Cleavable linkers	DX8953

CAT#	Product Name	Linker	Payload
ADC-AA-024	anti-MIgG(Fc)-C-DMDM ADC	Cleavable linkers	DMDM (duocarmycin DM)
ADC-AA-006	anti-HIgG(Fc)-C-DMDM ADC	Cleavable linkers	DMDM (duocarmycin DM)
ADC-AA-044	anti-Rat IgG(Fc)Fab-C-DMDM ADC	Cleavable linkers	DMDM (duocarmycin DM)
ADC-AA-013	anti-HIgG(Fc)Fab-C-DMDM ADC	Cleavable linkers	DMDM (duocarmycin DM)

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