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Protein G-MMAF ADC

Protein G-MMAF ADC (CAT#: ADC-AA-049)

This ADC product is comprised of a Protein G conjugated via a linker to MMAF. The MMAF is targeted to certain cancers by immunerecognition and delivered into cancer cells via receptor mediated endocytosis. Within the cell, MMAF binds to tubulins, interrupts microtubule dynamics, and subsequently, induces cell death. This product displays no obvious toxicity without a primary antibody and can be a quite efficient and economical alternative to pre-screening monoclonal antibodies as ADC candidates.

ADC Target
ADC Antibody
ADC payload drug

Name
IgG Fc

Overview
The fragment crystallizable region (Fc region) is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc region of IgG bears a highly conserved N-glycosylation site. Glycosylation of the Fc fragment is essential for Fc receptor-mediated activity. Fc binds to various cell receptors and complement proteins thus mediating different physiological effects of antibodies, such as opsonization, antibody dependent cellular cytotoxicity (ADCC), degranulation of mast cells, basophils, eosinophils and other processes.

Overview
Protein G

Name
MMAF (Monomethyl auristatin F)

Description
Derived from Auristatin,are water-soluble dolastatin analogs of dolastatin 10. Dolastatin 10 belongs to dolastatin family and it can powerfully bind to tubulin, thus inhibiting polymerization mediated through the binding to the vinca alkaloid binding domain, and causes cell to accumulate in metaphase arrest.

For Research Use Only. NOT FOR CLINICAL USE.

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Customer Reviews FAQ

Excellent

The Protein G-MMAF ADC from Creative Biolabs showed impressive efficacy in our cancer research. The targeted delivery of MMAF resulted in significant cell death with minimal off-target toxicity.

Excellent

Using the Protein G-MMAF ADC, we achieved excellent results in pre-screening monoclonal antibodies. The product was efficient and economical, providing reliable data in our ADC candidate evaluations.

Excellent

This ADC product exceeded our expectations with its targeted action and minimal toxicity. The Protein G-MMAF ADC was instrumental in advancing our cancer cell research, delivering consistent and potent results.

Excellent

Creative Biolabs' Protein G-MMAF ADC demonstrated strong binding affinity and effective cancer cell targeting. The product was crucial in our studies, offering a powerful tool for screening potential ADC candidates.

Excellent

The Protein G-MMAF ADC provided outstanding specificity in our experiments. The targeted delivery mechanism worked flawlessly, leading to significant inhibition of cancer cell proliferation in our assays.

Q: 1: What is the composition of the Protein G-MMAF ADC?
A: The Protein G-MMAF ADC is composed of Protein G conjugated via a linker to Monomethyl auristatin F (MMAF). MMAF is a cytotoxic agent that targets certain cancers through immune recognition and is delivered into cancer cells via receptor-mediated endocytosis, leading to cell death by disrupting microtubule dynamics.
Q: 2: How does MMAF function in the Protein G-MMAF ADC?
A: MMAF, or Monomethyl auristatin F, functions by binding to tubulins within the cancer cell, disrupting microtubule dynamics, and ultimately inducing cell death. This mechanism is specifically activated after the ADC is internalized into the target cells, making it a potent agent for targeted cancer therapy.
Q: 3: What is the role of Protein G in the Protein G-MMAF ADC?
A: Protein G in this ADC binds to the Fc region of antibodies, facilitating the targeting of MMAF to specific cancer cells. It allows the ADC to leverage the immune recognition of the Fc region, leading to receptor-mediated endocytosis of MMAF into cancer cells, where it exerts its cytotoxic effects.
Q: 4: What experimental considerations should be taken into account when using the Protein G-MMAF ADC?
A: When using this ADC, researchers should consider the specificity of Protein G to the Fc region of the antibody being studied. The concentration of the ADC and incubation times should be optimized to ensure efficient binding and internalization. Additionally, the absence of primary antibodies in controls is crucial to confirm the specificity of the cytotoxic effects.
Q: 5: Can the Protein G-MMAF ADC be customized for specific research needs?
A: Yes, Creative Biolabs offers customization services for the Protein G-MMAF ADC. Researchers can request modifications to the linker, conjugation process, or specific components to suit their experimental needs. This flexibility allows for tailored solutions that align with the specific objectives of the research.

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Same Target Same Payload

CAT#	Product Name	Linker	Payload
ADC-AA-053	Protein A-MMAF ADC		MMAF (Monomethyl auristatin F)
ADC-AA-048	Protein G-VC-MMAE ADC	VC (valine-citrulline)	MMAE (Monomethyl auristatin E)
ADC-AA-050	Protein G-MCC-DM1 ADC	MCC (Maleimidomethyl cyclohexane-1-carboxylate)	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-031	anti-MIgG(Fc)Fab-N-DM1 ADC	Noncleavable linkers	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-055	Protein A-Duocarmycin ADC		Duocarmycins

CAT#	Product Name	Linker	Payload
ADC-AA-019	anti-MIgG(Fc)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-001	anti-HIgG(Fc)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-W-491	Anti-TNFRSF17-Mc-MMAF ADC	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)
ADC-W-493	Anti-CD19 (clone huB4)-Mc-MMAF ADC	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)
ADC-W-485	Anti-EphA2 (clone 1C1)-Mc-MMAF ADC	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)

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