How does the anti-RIgG(HL)Fab-N-MMAF ADC minimize potential toxicity in the absence of a primary antibody?

The ADC displays minimal toxicity without a primary antibody due to the selective binding nature of the Fab fragment to rabbit IgG(H+L). This specificity ensures that the cytotoxic agent MMAF is only released in the vicinity of target cells, reducing the risk of damaging non-target cells.

What types of research applications are most suitable for using the anti-RIgG(HL)Fab-N-MMAF ADC?

This ADC is particularly suitable for applications involving the pre-screening of rabbit monoclonal antibodies to assess their potential as ADC candidates. It can be used in in vitro or in vivo models where rabbit IgG(H+L) is targeted.

What safety measures should be observed when handling the anti-RIgG(HL)Fab-N-MMAF ADC?

Due to the cytotoxic nature of MMAF, it is crucial to handle the ADC with care. Use appropriate personal protective equipment, work in a biosafety cabinet, and follow proper disposal protocols to avoid accidental exposure and environmental contamination.

What experimental controls are recommended when using the anti-RIgG(HL)Fab-N-MMAF ADC in laboratory studies?

It is recommended to include controls that lack the rabbit IgG(H+L) target to assess specificity and to use an isotype control ADC to evaluate non-specific binding and effects. These controls help in accurately interpreting the results of experiments involving this ADC.

Are there any specific considerations for using the anti-RIgG(HL)Fab-N-MMAF ADC in in vivo studies?

When using this ADC in animal models, it is important to ensure that the model expresses the target rabbit IgG(H+L) to evaluate the ADC's efficacy accurately. Monitoring for potential immune responses or toxicity in the animal model is also crucial to assess the safety profile of the ADC.

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anti-RIgG(HL)Fab-N-MMAF ADC

anti-RIgG(HL)Fab-N-MMAF ADC (CAT#: ADC-AA-037)

This ADC product is comprised of a Fab fragment of an anti-rabbit IgG(H+L) specific polyclonal antibody conjugated via a noncleavable linker to MMAF. The antibody portion is a Fab fragment of a secondary antibody and the drug portion, MMAF, is a cytotoxic small molecule which binds to tubulins, interrupts microtubule dynamics, and induces cell death. This product displays no obvious toxicity without a primary antibody and can be a quite efficient and economical alternative to pre-screening rabbit monoclonal antibodies as ADC candidates.

ADC Target
ADC Antibody
ADC Linker
ADC payload drug

Name
IgG(H+L)

Overview
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system, which is a protein complex composed of four peptide chains—two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. IgG is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses.

Overview
Fab fragment of anti-rabbit IgG(H+L) specific polyclonal IgG antibody

Species Reactivity
Rabbit

Name
Noncleavable linkers

Description
Noncleavable linkers, is considered noncleavable-meaning linker cleavage, and payload release does not depend on the differential properties between the plasma and some cytoplasmic compartments. Instead, the release of the cytotoxic drug is postulated to occur after internalization of the ADC via antigen-mediated endocytosis and delivery to lysosomal compartment, where the antibody is degraded to the level of amino acids through intracellular proteolytic degradation.

Name
MMAF (Monomethyl auristatin F)

Description
Derived from Auristatin,are water-soluble dolastatin analogs of dolastatin 10. Dolastatin 10 belongs to dolastatin family and it can powerfully bind to tubulin, thus inhibiting polymerization mediated through the binding to the vinca alkaloid binding domain, and causes cell to accumulate in metaphase arrest.

For Research Use Only. NOT FOR CLINICAL USE.

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Customer Reviews FAQ

Excellent

Impressive Specificity and Efficacy

The anti-RIgG(HL)Fab-N-MMAF ADC from Creative Biolabs precisely targets and destroys tubulin dynamics without off-target effects. Perfect for pre-screening applications

Excellent

A Game Changer for ADC Screening

Using this ADC, we've accelerated our antibody development with its efficient targeting and cytotoxic capabilities. No primary antibody toxicity is a huge plus

Excellent

High-Performance in Lab Tests

The anti-RIgG(HL)Fab-N-MMAF ADC demonstrates exceptional cell death induction, significantly advancing our research into antibody-mediated cytotoxicity.

Excellent

Reliable and Robust

This ADC has proven invaluable in our studies, providing consistent results without toxicity issues. It's an economical solution for rabbit monoclonal antibody screening.

Excellent

Outstanding Results with No Complications! The specific action of the anti-RIgG(HL)Fab-N-MMAF ADC on microtubules is precise and highly effective. A reliable choice for complex immunological research.

Q: 1: How does the anti-RIgG(HL)Fab-N-MMAF ADC minimize potential toxicity in the absence of a primary antibody?
A: The ADC displays minimal toxicity without a primary antibody due to the selective binding nature of the Fab fragment to rabbit IgG(H+L). This specificity ensures that the cytotoxic agent MMAF is only released in the vicinity of target cells, reducing the risk of damaging non-target cells.
Q: 2: What types of research applications are most suitable for using the anti-RIgG(HL)Fab-N-MMAF ADC?
A: This ADC is particularly suitable for applications involving the pre-screening of rabbit monoclonal antibodies to assess their potential as ADC candidates. It can be used in in vitro or in vivo models where rabbit IgG(H+L) is targeted.
Q: 3: What safety measures should be observed when handling the anti-RIgG(HL)Fab-N-MMAF ADC?
A: Due to the cytotoxic nature of MMAF, it is crucial to handle the ADC with care. Use appropriate personal protective equipment, work in a biosafety cabinet, and follow proper disposal protocols to avoid accidental exposure and environmental contamination.
Q: 4: What experimental controls are recommended when using the anti-RIgG(HL)Fab-N-MMAF ADC in laboratory studies?
A: It is recommended to include controls that lack the rabbit IgG(H+L) target to assess specificity and to use an isotype control ADC to evaluate non-specific binding and effects. These controls help in accurately interpreting the results of experiments involving this ADC.
Q: 5: Are there any specific considerations for using the anti-RIgG(HL)Fab-N-MMAF ADC in in vivo studies?
A: When using this ADC in animal models, it is important to ensure that the model expresses the target rabbit IgG(H+L) to evaluate the ADC's efficacy accurately. Monitoring for potential immune responses or toxicity in the animal model is also crucial to assess the safety profile of the ADC.

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Same Target Same Linker Same Payload

CAT#	Product Name	Linker	Payload
ADC-AA-047	anti-Rat IgG(HL)Fab-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-034	anti-RIgG(HL)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-036	anti-RIgG(HL)-C-MMAE ADC	Cleavable linkers	MMAE (Monomethyl auristatin E)
ADC-AA-066	Anti-Rat IgG(H+L)-C-PBD ADC	Cleavable linkers	PBD (pyrrolobenzodiazepine)
ADC-AA-046	anti-Rat IgG(HL)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)

CAT#	Product Name	Linker	Payload
ADC-AA-034	anti-RIgG(HL)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-045	anti-Rat IgG(HL)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-031	anti-MIgG(Fc)Fab-N-DM1 ADC	Noncleavable linkers	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-039	anti-Rat IgG(Fc)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-041	anti-Rat IgG(Fc)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)

CAT#	Product Name	Linker	Payload
ADC-AA-002	anti-HIgG(Fc)-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-010	anti-HIgG(Fc)Fab-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-027	anti-MIgG(Fc)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-W-493	Anti-CD19 (clone huB4)-Mc-MMAF ADC	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)
ADC-AA-028	anti-MIgG(Fc)Fab-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)

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