Anti-Membrane Protein Antibody Production by Dendritic Cell Antigen Presentation

With excellent expert teams and rich experience in antibody discovery of membrane protein, Creative Biolabs has proudly serviced numerous worldwide customers and established good relations of cooperation. With non-stop efforts to serve our clients, now, we offer cutting-edge service to produce anti-membrane protein antibody by dendritic cells as immunogen format. This is a world-leading anti-membrane protein antibody production technology with antigens presented on the surface of dendritic cells. Our service would contribute to the growing interest in targeting membrane proteins as therapeutic targets with antibodies.

Anti-membrane protein antibodies show valuable application in clinical therapies due to the irreplaceable roles of membrane proteins in lots of physical activities. However, the production of membrane proteins is quite challenging for their complicated structures and low abundance in nature. Thus, the discovery process of anti-membrane protein antibodies is hampered by the immunogen preparation of membrane proteins. Moreover, even the immunogen preparation problem is solved, in some cases, the immunogen fails to generate enough immune response in animals and it still doesn’t work well for antibody discovery. To overcome the problems, talented scientists from Creative Biolabs have proudly developed a new immunogen format, antigens presented on dendritic cells, which skips the difficulties of membrane protein purification and reconstitution but still has powerful immunogenic effect in animals.

Dendritic cells are a heterogeneous population of antigen-presenting cells that have crucial roles in promoting both pro- and anti-inflammatory immune responses. They are necessary for activation of naive T cells. Dendritic cells present antigen to both helper and cytotoxic T cells. They can also perform cross-presentation, a process by which they present exogenous antigen on MHC class I molecules to cytotoxic T cells. Cross-presentation allows for the activation of these T cells. Meanwhile, dendritic cells also play a role in peripheral tolerance, which contributes to prevention of auto-immune disease. With membrane proteins over-expression in dendritic cells, membrane proteins are on the cell surface as the same in other mammalian cell lines. They fold to their native structure conformation in native membrane environment. It has been successfully applied to mice immune process with several GPCR members.

Fig 1 Schematic representation of dendritic cells functions. (Hubo M, et.al. 2013)

Highlight advantages of Anti-Membrane Protein Antibody Production by Dendritic Cell Antigen Presentation Service:

No sequence modification is necessary
No complicated purification process is needed
Membrane proteins in native structure
Native membrane environment
Outstanding ability to cause strong immune responses
Time-saving
Minimal cytotoxicity

With rich experience for hundreds of successful cases by Magic™ platform, Creative Biolabs devotes itself to offer time- and labor-saving membrane protein services to all customers based on your timeline and budget. As alternatives, we also have membrane protein antigen types including membrane protein reconstitution into liposomes, membrane protein incorporation into nanodiscs, membrane protein incorporation into polymers, whole cell antigens, membrane protein-carrying exosomes and virus-like particles. All of these antigen formats can be used in our anti-membrane protein antibody discovery service, such as anti-membrane protein antibody discovery using hybridoma, phage display immune library technology. Please feel free to contact us for more information.

Reference

Hubo M, Trinschek B, Kryczanowsky F, et al. (2013). Costimulatory molecules on immunogenic versus tolerogenic human dendritic cells. Frontiers in Immunology. 4, 1-14.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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