Anti-Membrane Protein Antibody Production Using Designed Peptides

Creative Biolabs has long been a forward-looking research institute as well as a high-end customer service provider. With years of experience in antibody discovery of membrane protein, we have served numerous worldwide clients with satisfactory products. Now, we introduce our professional membrane protein peptide design service for anti-membrane protein antibody production. This is a convenient and smart strategy compared with expression and purification of full-length membrane proteins aiming to accelerate your research progress.

Integral membrane proteins, including GPCRs and ion channels, are characterized by multiple transmembrane spanning domains. These proteins represent large protein families and there is an estimation that approximately 350 non-olfactory GPCRs and 400 annotated ion channel genes are in human genome. The value of these proteins is illustrated by the success of using small molecule drugs to target these protein classes. Besides, there are reports suggest that GPCRs and ion channels consist of the second and third largest biochemical classes targeted by marketed small molecule drugs. However, it is also recognized that small molecule drugs face issues which hamper their progress in the clinic relating to toxicity and lack of target selectivity. Given this situation, anti-membrane protein antibodies show appealing application for their higher affinity, higher specificity, and lower cytotoxicity. The discovery process of anti-membrane protein antibody always starts with the preparation of target membrane protein as immunogen or antigen. However, the structures of membrane proteins are complicated. Besides, the expression and purification of full-length membrane proteins sometimes are technically difficult even for experienced experts. In order to get rid of the problems, based on structure biology, we could design effective peptides from the extracellular or intracellular loops of target membrane proteins to mimic the full-length protein functions and activities.

Well-designed peptide antigens are capable of raising functional antibodies that recognize the native conformation of many multi-pass integral membrane protein antigens with higher specificity, accessibility, and lower toxicity. Meanwhile, during downstream selection, well-designed peptide can also work effectively to identify desired binders.

Highlight advantages of Anti-Membrane Protein Antibody Production Using Designed Peptides

Higher solubility and stability compared with full-length proteins
High affinity and specificity
No complicated purification and reconstitution processes are needed
Time-saving
Detergent-free and lower cytotoxicity
Delicate consideration and flexible region choices

With rich experience for hundreds of successful cases, Creative Biolabs devotes itself to offer high-quality membrane protein for immunogens and selections during antibody discovery. As alternatives, we also have membrane protein formats for functional applications such as whole cell antigen presentation and exosome immunogens. Please feel free to contact us for more information.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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