ASPH Membrane Protein Introduction

Introduction of ASPH

Aspartyl/asparaginyl beta-hydroxylase (HAAH) is an enzyme that in humans is encoded by the ASPH gene. ASPH, a ~86KD type II transmembrane protein, is a member of the α-ketoglutarate-dependent dioxygenase family. It catalyzes the β-hydroxylation of aspartyl and asparaginyl residues in epidermal growth factor (EGF)-like repeats of various proteins such as Notch.

Basic Information of ASPH
Protein Name	Aspartyl/asparaginyl beta-hydroxylase
Gene Name	ASPH
Aliases	Aspartate beta-hydroxylase, ASP beta-hydroxylase, Peptide-aspartate beta-dioxygenase, BAH
Organism	Homo sapiens (Human)
UniProt ID	Q12797
Transmembrane Times	1
Length (aa)	758
Sequence	MAQRKNAKSSGNSSSSGSGSGSTSAGSSSPGARRETKHGGHKNGRKGGLSGTSFFTWFMVIALLGVWTSVAVVWFDLVDYEEVLGKLGIYDADGDGDFDVDDAKVLLGLKERSTSEPAVPPEEAEPHTEPEEQVPVEAEPQNIEDEAKEQIQSLLHEMVHAEHVEGEDLQQEDGPTGEPQQEDDEFLMATDVDDRFETLEPEVSHEETEHSYHVEETVSQDCNQDMEEMMSEQENPDSSEPVVEDERLHHDTDDVTYQVYEEQAVYEPLENEGIEITEVTAPPEDNPVEDSQVIVEEVSIFPVEEQQEVPPETNRKTDDPEQKAKVKKKKPKLLNKFDKTIKAELDAAEKLRKRGKIEEAVNAFKELVRKYPQSPRARYGKAQCEDDLAEKRRSNEVLRGAIETYQEVASLPDVPADLLKLSLKRRSDRQQFLGHMRGSLLTLQRLVQLFPNDTSLKNDLGVGYLLIGDNDNAKKVYEEVLSVTPNDGFAKVHYGFILKAQNKIAESIPYLKEGIESGDPGTDDGRFYFHLGDAMQRVGNKEAYKWYELGHKRGHFASVWQRSLYNVNGLKAQPWWTPKETGYTELVKSLERNWKLIRDEGLAVMDKAKGLFLPEDENLREKGDWSQFTLWQQGRRNENACKGAPKTCTLLEKFPETTGCRRGQIKYSIMHPGTHVWPHTGPTNCRLRMHLGLVIPKEGCKIRCANETKTWEEGKVLIFDDSFEHEVWQDASSFRLIFIVDVWHPELTPQQRRSLPAI

Function of ASPH Membrane Protein

ASPH is a highly conserved enzyme, which catalyzes the hydroxylation of aspartyl and asparaginyl residues in epidermal growth factor-like domains of proteins including Notch and homologs. It was originally described as overexpressed in HCC and cholangiocellular carcinoma. Currently, ASPH has been found upregulated in more than 20 tumor types, such as HCC, CCC, pancreatic, colon, breast and non-small cell lung cancer. Overexpression of ASPH was found in HCC with a malignant phenotype characterized by increased cell motility, invasion, and metastasis. ASPH has been reported to mediate cell migration via hydroxylation-dependent activation of Notch signaling networks.

ASPH Membrane Protein Introduction Fig.1 Schematic representation of proposed role of ASPH in key signaling pathways responsible for infiltrative spread of GBM. (Sturla, 2016)

Application of ASPH Membrane Protein in Literature

Sturla L.M., et al. Aspartate-β-hydroxylase (ASPH): A potential therapeutic target in human malignant gliomas. Heliyon. 2016, 2(12): e00203. PubMed ID: 27981247

GBM treatment strategies could be made more effective by including small molecule inhibitors of ASPH.
Iwagami Y., et al. Aspartate β-hydroxylase modulates cellular senescence through glycogen synthase kinase 3β in hepatocellular carcinoma. Hepatology. 2016, 63(4): 1213-1226. PubMed ID: 26683595

ASPH enzymatic activity is a novel therapeutic target for hepatocellular carcinoma.
Tomimaru Y., et al. Aspartate-β-hydroxylase induces epitope-specific T cell responses in hepatocellular carcinoma. Vaccine. 2015, 33(10): 1256-1266. PubMed ID: 25629522

ASPH protein and related peptides were highly immunogenic in patients with HCC and produce the type of cellular immune responses required for generation of anti-tumor activity.
Dong X., et al. Aspartate β-Hydroxylase expression promotes a malignant pancreatic cellular phenotype. Oncotarget. 2015, 6(2): 1231-1248. PubMed ID: 25483102

Critical involvement of ASPH in PC growth and progression, provide new insight into the molecular mechanisms leading to tumor development and growth and have important therapeutic implications.
Patel N., et al. Mutations in ASPH cause facial dysmorphism, lens dislocation, anterior-segment abnormalities, and spontaneous filtering blebs, or Traboulsi syndrome. Am J Hum Genet. 2014, 94(5): 755-759. PubMed ID: 24768550

ASPH-knockout mice had a foreshortened snout, which corresponds to the facial abnormalities in individuals with Traboulsi syndrome.

ASPH Preparation Options

To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-ASPH antibody development services.

Creative Biolabs' skillful scientists are glad to leverage our expertise and advanced technologies to help you with the member protein research. If you are interested, please feel free to contact us for more details.

Reference

Sturla L M, et al. (2016). Aspartate-β-hydroxylase (ASPH): A potential therapeutic target in human malignant gliomas. Heliyon. 2(12), e00203.

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