This product is a B cell engineering plasmid designed by genomic integration of B cell receptors (Luc-CAG-Sec/SPI-CD79α-CD28-Myc) and CRISPR-Cas (Cas13d-dsDNA-RuvC, Nuc-NNNNGATT-attP) systems. This product can precisely introduce cassettes of several kb size into the genome of various B cells to enhance surface expression and antigen recognition of a synthetic BCRs.
| Clone | BGPIX-3315 |
| Host | Mouse |
| Targeting Cell Type | Raji-Luc2 |
| Reporter gene | Luc |
| Promoter | CAG |
| Signal peptides | Sec/SPI |
| Signaling domains | CD79α |
| TM domains | CD28 |
| Detection tags | Myc |
| Linkers | Flexible Linker |
| Co-Receptors | LILRB |
| Vector Type | Lentivirus |
| Vector Name | BGpCDH2 |
| Class | 2 single-Cas |
| Type | IV |
| Subtype | B |
| Effector | Cas13d |
| DNA Type | dsDNA |
| Nuclease domains | RuvC, Nuc |
| TracrRNA requirement | No |
| PAM/PFS | NNNNGATT |
| Insertion | attP |
| Construction | Luc-CAG-CD79α-Cas13d-dsDNA-attP |
| Target | SALL2 |
| Alternative Names | SALL2; COLB; HSAL2; Sal-2; ZNF795; p150(Sal2); spalt-like transcription factor 2; spalt like transcription factor 2 |
| Format | Liquid/lyophilized |
| Shipping | It is usually shipped overnight at room temperature in small cardboard boxes. If hot temperatures are expected during transit, they will be shipped in polystyrene containers with gel ice packs. |
| Handling Advice | No special measures are necessary. Keep away from food, drink and animal feedingstuffs. |
| Storage | Plasmid (lyophilized), short-time storage at Room Temperature, recommended store at -20°C or -80°C ; Plasmid (Liquid), blue ice store at 4°C. |
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