Introduction
One of the most commonly-used nonhuman primate species, not only in toxicology but also other biomedical research fields, particularly neuroscience and infectious disease studies is Cynomolgus (Macaca fascicularis), known as crab-eating macaque. PBMC or peripheral blood mononuclear cells, which consist of lymphocytes (T-lymphocytes / T-cells, B-lymphocytes/B-cells and natural killer cells) and monocytes occupy a central position in immunological studies. In this blog, you will learn about the latest scientific research and uses of Cynomolgus Monkey PBMCs.
Recent Scientific Studies on Cynomolgus Monkey PBMCs
Effect of Cryopreservation on PBMCs: A recently study reported the effects of long-term cryopreservation on PBMCs using flow cytometry. Results showed significantly decreased cell recovery and viability with cryopreservation, the PBMCs were still within normal ranges after prolonged storage (6 months). The authors suggest that “cryopreserving samples would represent a reliable resource for clinical protocols and research projects”
Immune cell heterogeneity and dynamics: Single-cell transcriptomic and regulomic mapping researches exposed a high level of heterogeneous trends in immune cells among Cynomolgus monkeys. One such study identified 72,284 immune-related cells from different tissues and organs that were sub-grouped into 13 major clusters depending on the gene signature. This tissue-specific immune cell atlas elucidates the roles of these cells along with underlying intercellular crosstalk that are essential for developing targeted therapies (Home).
Commercial Availability and Uses
Cynomolgus Monkey PBMCs are sold commercially for various research uses. Fresh whole blood is separated and density centrifuged to isolate these cells. They are essential especially for experiments in toxicology, pharmacology and immunology because of their pivotal favorable role with the cell mediated immunity or even humoral immunity. They are also adapted to tests and assays like cell-cell interaction, expressions analysis (PCR), immunofluorescent staining etc., showing them as a multipurpose in scientific research.
Main Applications of Cynomolgus Monkey PBMC Immunological Studies: Due to the functional similarities between them with human immune cells, Cynomolgus Monkey PBMCs have proven useful in immunological research. They are harnessed for investigative study of immune responses, such as T cell activation and proliferation, cytokine profile production, regulatory functional aspects. This is facilitating our knowledge about the immune mechanisms and advancing immunotherapeutic strategies.
Drug Development and Toxicology
Because Cynomolgus monkeys are so genetically close to humans, their PBMCs are highly suitable for preclinical drug testing and toxicological risk assessment. These cells are a type of cell that researchers use to test whether new drugs (especially immunotherapies) work and which ones might be safest.
Disease Modeling: In addition to autoimmunity, infectious diseases and cancer are examples of other fields where such PBMC-based disease models exist. Since PBMCs are easily collected from healthy and diseased animals, they provide an excellent choice for studying disease mechanisms of interest by gaining insights into potential therapeutic targets using this non-invasive model.
Conclusion
Cynomolgus Monkey PBMCs are a vital resource across biomedical research, helping researchers to study immune function and disease mechanisms. Continuing progress on cryopreservation, single-cell analysis and increased commercial availability further widens their utility in scientific research. These PBMCs will remain a vital part of the process for new therapies and our full understanding of human health and disease as research continues.
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Rhesus Monkey PBMCs (NHP-PC002)
Immortalized GFP-Expressing NHP Cell Products