Yeast Hybrid related Auxiliary Reagents

Deep plowing in the field of biomolecule interaction for many years, Creative Biolabs has won a good reputation and rich experience from our thousands of projects. In terms of yeast hybrid-based interaction screening and validation, we provide both one-stop/custom Yeast Hybrid services and high-quality products to global researchers.

Yeast Hybrid Overview

Yeast hybrid assays are powerful tools used to study various types of biomolecular interactions in a cellular context. Currently, three types of yeast hybrid assays have been widely used:

Yeast Two-Hybrid (Y2H) Assay: This assay is mainly used to study protein-protein interactions, which is developed by fusing a bait protein to the DNA-binding domain (DBD) of yeast transcriptional factor and a prey protein to an activation domain (AD). When the bait interacts with prey protein, they approach and reconstitute a functional transcription factor that activates reporter genes.

Yeast One-Hybrid (Y1H) Assay: This assay is used to study protein-DNA interactions. It involves a DNA sequence of interest (bait) and a protein of interest (prey) fused to a transcriptional activation domain. When the prey protein binds to the bait DNA sequence, it activates the expression of a reporter gene.

Yeast Three-Hybrid (Y3H) Assay: This assay is used to study RNA-protein interactions. It involves an RNA sequence (bait), a protein of interest (prey) fused to a transcriptional activation domain, and a bridging protein. When the prey protein binds to the RNA bait sequence and the bridging protein interacts with both, it activates the expression of a reporter gene. Moreover, Y3H is also often used to detect the interaction between three proteins, that is, the influence of mediator proteins on the interaction between a pair of proteins.

Fig. 1 Y3H for interaction detection between three proteins. Fig 1. Schematic representation of the bacterial three-hybrid system.¹

Product List

Starting from yeast hybrids, Creative Biolabs offers a full range of pre-made products for different yeast hybrid assays, including Y1H, Y2H, and Y3H. Please directly contact us if your products of interest are not on the list, our technical team will support you with tailored solutions and products as soon as possible.

Reference

Tharad, M., Samuchiwal, S. K., Bhalla, K., Ghosh, A., Kumar, K., Kumar, S., & Ranganathan, A. (2011). A three-hybrid system to probe in vivo protein-protein interactions: application to the essential proteins of the RD1 complex of M. tuberculosis. PLoS One, 6(11), e27503. Distributed under open access license CC BY 4.0, without modification.

Cat	Product Name	Applications	Size	Datasheet	Inquiry
HN-0125-TT1	Saccharomyces cerevisiae Competent cell Preparation Kit	Yeast hybrid	100T/200T		Inquiry
HN-0125-TT2	Sorbitol Solution (filtered and sterilized)	Yeast hybrid	100ml		Inquiry
HN-0125-TT3	TE/LiAC Yeast Competent Preparation Solution	Yeast transformation	30ml/100ml		Inquiry
HN-0125-TT4	PEG/LiAC Yeast Transformation Solution	Yeast transformation	50ml/100ml		Inquiry
HN-0125-TT5	3-amino-1,2,4-triazole (3-AT) Solution	Yeast hybrid	100ml		Inquiry
HN-0125-TT6	3-amino-1,2,4-triazole (3-AT)	Yeast hybrid	5g/25g		Inquiry
HN-0125-TT7	X-α-Gal Solution	Yeast hybrid	1ml/5×1ml/10×1ml		Inquiry
HN-0125-TT8	X-α-Gal	Yeast hybrid	250mg/5×250mg/500mg/1g		Inquiry
HN-0125-TT9	50%PEG Solution	Yeast transformation	50ml		Inquiry
HN-0125-TT10	Carrier DNA	Yeast transformation	1ml/10×1ml		Inquiry
HN-0125-TT11	Yeast Colony Lysis Buffer	Yeast hybrid	1ml/2×1ml/5×1ml/10×1ml		Inquiry
HN-0125-TT12	2×Yeast direct PCR Mix (for pYES2)	Yeast hybrid	1ml/5ml		Inquiry
HN-0125-TT13	2×Yeast direct PCR Mix (for pAbAi)	Yeast hybrid	1ml/5ml		Inquiry
HN-0125-TT14	2×Yeast direct PCR Mix (for pPR3-N)	Yeast hybrid	1ml/5ml		Inquiry
HN-0125-TT15	2×Yeast direct PCR Mix (pGBKT7)	Yeast hybrid	1ml/5ml		Inquiry
HN-0125-TT16	2×Yeast direct PCR Mix (for pGADT7)	Yeast hybrid	1ml/5ml		Inquiry
HN-0125-TT17	2×Yeast direct PCR Mix (no primer)	Yeast hybrid	1ml/2×1ml/5ml/2×5ml/5×5ml		Inquiry
HN-0125-TT18	One-step Yeast Transformation Kit	Yeast transformation	50T		Inquiry
HN-0125-TT19	Yeast Genomic DNA Extraction Kit	Yeast gDNA extraction	50T /100T		Inquiry
HN-0125-TT20	cDNA Normalization Kit	Yeast hybrid library preparation	10T		Inquiry

All listed customized services & products are for research use only, not intended for pharmaceutical, diagnostic, therapeutic or any in vivo human use.

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