This product is an ELISA kit for the determination of Dog Complement Factor B in the samples of Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate. It is highly sensitive and available for the detection of Dog Complement Factor B as low as 1.0 pg/mL.
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Assay Type | Competition ELISA |
Sensitivity | 1.0 pg/mL (100-2500 pg/mL) |
Pack Size | 96 tests |
Species Reactivity | Canine |
Target | Factor B |
Applications | ELISA |
Storage | 4°C |
Introduction | Complement factor B is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. It also involves in cleavage of Arg-|-Ser bond in complement component C3 alpha-chain to yield C3a and C3b, and Arg-|-Xaa bond in complement component C5 alpha-chain to yield C5a and C5b. |
I wouldn't hesitate to recommend itExcellentThe assay provides precise and reliable quantification of Factor B, which is essential for our disease model studies. The kit includes all necessary components, and the instructions are clear and concise. The quality of the antibodies used in the kit is exceptional, ensuring high specificity and minimal background noise. The standard curve is easy to generate, and the results are reproducible across different batches.
This kit is definitely worth the investment for any lab studying canine complement factorsExcellentWe have used this kit on various sample types, including serum and plasma, and it has consistently provided reliable and accurate results. The customer support from the manufacturer is also commendable, offering prompt assistance whenever we had queries.
Using the ELISA kit has significantly streamlined our research workflowExcellentWe appreciate the thorough instructions and troubleshooting tips provided in the manual, which have helped us optimize our assays. The results we have obtained are consistent and reproducible, which is critical for our longitudinal studies.
We plan to continue using it for future projectsExcellentOur lab recently switched to the ELISA kit, and we couldn't be happier with the results. The kit's assay protocol is straightforward, making it accessible even for less experienced technicians. The reagents provided are of high quality, and the assay's sensitivity allows us to detect low levels of Factor B with confidence.