Neural Stem Cell
The therapeutic genes can be introduced into cells by transfection or transduction used for gene therapy. The development of somatic cell reprogramming methods provides an attractive method of autologous cells for transplantation and treatment of degenerative diseases, and the therapeutic method no longer depends on targeting to specific cell types in patients. Gene delivery to cells ex vivo circumvents the limitation of host immunity and incompatibility. Neural stem cells (NSCs) are well suited as vehicles for the transfer of therapeutic genes in a wide array of clinical settings. Creative Biolabs provides cutting-edge cell therapy research services covering every stage of NSC development, including isolation, expansion, characterization, and differentiation.
Neural stem cells (NSCs) are self-renewing stem cells and have the capacity to differentiate into mature neuronal and glial cell types of the nervous system in developing brain. NSCs are already being used as gene delivery tools for rescuing neurons lost to injury or disease. Their potential to differentiate and genetic plasticity make them the modality of choice for cellular transplantation. Neural stem cells (NSCs) are the main vehicle for genetic and molecular therapies in the central nervous system (CNS) that have been applied to treat stroke, Parkinson's disease, Huntington's disease and multiple sclerosis.
Neural Stem Cells as Vehicles for Gene Therapy
NSCs migrate towards the specified site of cell damage to repair or replace dying neural cells. Reprogram patient-specific human NSCs can improve delivery of therapeutic NSCs in the brain. NSCs undergo the processes of isolation, proliferation, genetic manipulation and differentiation in vitro and then reintroduce into developing or pathologically altered CNS. Creative Biolabs provides viral and non-viral vectors to introduce therapeutic genes into adult neural stem cells efficiently, including adenoviral and VSV-G-pseudotyped retroviral vectors.
Fig.1 Cardinal neural stem cell properties
NSCs can be isolated from a number of fetal brain regions, including the ventricular zone, cortex, striatal anlage, midbrain, and spinal cord, and propagated in vitro by a variety of equally effective and safe means. Based on the different diseases, various therapeutic genes have been inserted into the NSCs to delivery functional proteins for increasing the selective targeting potential of NSCs, as well as stimulating regulation for cell repairment. Mouse models of CNS diseases have already demonstrated the efficacy of such NSC-mediated treatment, and NSCs as vehicles for gene therapy have enormous potential on clinical treatment.
Advantages
- Inherent long-distance migratory capabilities
- Remarkable capacity to integrate into brain structures
With a skilled team of scientists, Creative Biolabs provides the state-of-the-art stem cells platform for basic research and preclinical applications to develop neural stem cell as vehicle for gene delivery system. In addition, we utilize cell and membrane engineering techniques to increase the efficiency and selective targeting potential of neural stem cell. We also offer numerous methods of delivery genetic material to choose based on the desired duration of the genetic modification both in vivo and ex vivo, including transiently expressed and stably expressed of therapeutic genes. If you are interested in the study of gene therapy using stem cells to delivery useful genes, please don't hesitate to contact us and we will do our best to satisfy your needs.
Reference
- Jandial, R.; et al. (2008). Genetic modification of neural stem cells. Molecular Therapy, 16(3), 450-457.
