Adeno-associated Virus Titration

Creative Biolabs is able to provide state-of-art services for any adeno-associated virus (AAV) vector project with qualitative measurements. Our professional team will provide flexible solutions for AAV vector generation, titration testing, and safety evaluation. We are also specialized in improving current AAV vectors for gene therapy, which can boost long term efficacy and reduce the cost to a more affordable level.

Introduction of the Titration of AAV Vectors

AAV vectors have been regarded as attractive tools for gene therapy. However, a variety of clinical trials have demonstrated that AAV vectors are associated with severe adverse reactions in patients. The exact amount of AAV vectors should be quantified in human disease treatments. As a result, a wide collection of strategies has been developed to detect the titration of produced vectors, such as quantitative PCR (qPCR), enzyme-linked immunosorbent assay (ELISA), as well as dot-blot assays. Pilot studies have shown that ELISA can be widely used to detect the number of viral capsids that contain genetic materials. The data suggest that it is an accurate and reproducible way for AAV vector titration. Meanwhile, scientists have revealed that dot-blot and qPCR are reliable methods for determining the titration of different AAV vectors, among them, qPCR is the most rapid and high-throughput assay to titer AAV vectors in pre-clinical studies. For instance, more recent studies have reported that the infectious titers of recombinant AAV-2 (rAAV-2) particles can be identified by a novel qPCR assay. Moreover, this method has been validated by using the infectious center assay (ICA) and flow cytometry (FACS) assay.

Figure 1. Titration Service of AAV Vector.

Service

AAV vectors are efficient gene delivery systems for stably transferring targeted genes into a range of human cells. Many attempts have been made to improve the gene expression and safety of AAV vectors, thereby leading to the approval of these vectors for clinical studies in humans. As a consequence, a number of methods have been generated to detect the titer of AAV vectors, the level of gene expression, as well as the effectiveness of gene transduction. Currently, Creative Biolabs has established a battery of assays for in-depth analysis of AAV vector titers and expression profiles of candidate genes, including Titration of rAAV Genome Copy Number Assay, Infectious Titration Assay, and Genome Titration Assay. Equipped with state-of-art technologies, we have successfully accomplished several projects of AAV vector titration for our worldwide customers. As noted, the copy number of a recombinant AAV vector genome has been detected in infected cells by using our novel qPCR technology. In general, a set of TaqMan PCR primers and probes will be designed for detecting the AAV genome in the coding region.

As the long-term pioneer as well as the market leader in the field of gene therapy, Creative Biolabs has successfully established and commercialized a wide spectrum of AAV vector-based services for a wide variety of disease treatment. With a variety of mature and comprehensive platforms, we are committed to conducting cutting-edge research projects, developing perfect AAV vectors, and meeting critical medical needs. If you are interested in our services, please contact us for more details.