Immortality of the hybridoma cell line is a property of myeloma cells which are fused to antibody-specific B cells. If your hybridoma no longer grows well, re-fusing a hybridoma to new myeloma cells can help. Scientists at Creative Biolabs have extensive experience in hybridoma generating. After years of unremitting efforts, we offer hybridoma re-fusing services for global clients. Creative Biolabs will be your strong support on your way to hybridoma rescue.
Cell fusion is to fuse two types of cells into one via natural or artificial means, obtaining fusion cells that exhibit characteristics of both cells. Cell fusion technology plays a critical role in modern biotechnology, especially in the production of monoclonal antibodies (mAbs), which requires the fusion of antibody-producing cells with continuously dividing cancer cells, forming into hybridoma cells.
Fig.1 Schematic of cell fusion platform.1
As a conventional method to isolate mAbs, hybridoma technology is still being widely utilized currently, because of the significant advantage that once hybridoma cell clones are established, mAbs can be produced continuously. The method fuses cells that can indefinitely proliferate with antigen-specific B cells, making B cells easier to be cultured and able to persistently secrete antibodies. However, previous cytogenetic studies have revealed that the chromosome from one parent is highly likely to be lost during continuous proliferation, resulting in poor growth, diminished levels of interested protein expression, and a decrease in the quality of products. Besides, improper methods, inaccurate operation in preparation, or inappropriate storage can also affect the activity of hybridoma and the efficiency of mAb products. Therefore, timely hybridoma re-fusion can be a solution to remedy these conditions.
Hybridoma re-fusion is to fuse hybridoma cells with myeloma cell lines to obtain a new monoclonal cell line, and select the most suitable cell line among the new monoclonal cell lines for further use in mAb production. It has been recognized as an appropriate method to deal with chromosome loss by increasing the corresponding parental chromosome complement, aiding in rescuing hybridoma cells, and restoring their activity.
In the whole process of hybridoma preparation, the selection of the optimal myeloma cells is especially critical for achieving the desired hybridoma. Creative Biolabs will re-fuse your hybridoma to two different myeloma cell lines and establish new monoclonal cell lines for you that are selected for optimal growth as well as antibody production.
A: Generally, 1~2 mg target protein is required to test parental re-fusion cultures and subsequent subclones.
A: Collected supernatant samples will be sent to our clients for testing.
Equipped with years of experience in hybridoma technology, along with novel tech platforms and an expert team, Creative Biolabs is capable of assisting you in your projects with reliable hybridoma rescue services including but not limited to hybridoma re-fusion. Please feel free to contact us for inquiries into your customized services.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
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