Nowadays, many microbiota classification and metagenomic analyses are based on the bacterial 16S ribosomal RNA (16S rRNA) gene. Creative Biolabs offers a comprehensive 16S rRNA sequencing service for the identification and classification of species in gut microbiota samples.

16S rRNA Gene

16S rRNA gene, coding for 16S rRNA, are used in reconstructing phylogenies, due to the slow rates of evolution of this region of the gene. The 16S rRNA gene is a suit target gene for sequencing DNA in samples containing up to thousands of different species, because it is a highly conserved component of the transcriptional machinery of all DNA-based life forms. Moreover, the 16S rRNA gene is composed of both variable and conserved regions. The conserved region makes universal amplification possible and the sequencing of variable regions allows discrimination between specific different microorganisms such as bacteria, archaea and microbial eukarya.

Fig.1 Structure of Ribosomal RNA. (Wikipedia)

16S rRNA Sequencing

16S rRNA sequencing is commonly used for the identification, classification, and quantitation of microbes within complex biological mixtures including environmental samples (marine water) and gut samples (gut microbiome). Using 16S rRNA sequencing, you will get an overview of the community composition of a microbiome, identify differences between groups and variation within groups for a fast and affordable investigation of any microbial community.

16S rRNA sequencing is a type of amplicon sequencing. In detail, all or parts of the gene from microbiota samples are amplified with universal primers specific to the conserved regions. The primer sets are designed to capture the widest range of different microorganisms possible and then the amplified fragments are analyzed via next-generation sequencing (NGS).

Applications of 16S rRNA Sequencing

• Study complex microbiomes
• Identify low-abundance bacteria
• Characterize non-cultivable bacteria
• Profile hundreds of microorganisms from a single analysis
• Semi-quantify the relative abundance of microbiome members
• Provide faster and more accurate classification than traditional identification methods
• Multiplex microbial samples, providing more cost-effective microbiome characterization

Our Services

• Sample preparation
  Creative Biolabs offers the extraction process from human, and animal gut microbiota samples, performed at highly controlled and traceable conditions in our advanced and certified laboratory.
• Sequencing
  16S rRNA sequencing is performed using our advanced platform with the reagent kit.
• Data analysis
  Creative Biolabs offers 16S bioinformatics pipelines which can be customized to suit your needs:
• Bacterial abundance table using ASVs or OTUs
• Taxonomic profiles (genus/species resolution)
• Biostatistics linking the microbiome to clinical outcomes
• Publication ready figures and statistics
• Integration with study-specific datasets
• Custom solutions upon request
• Publication ready results
  Creative Biolabs delivers your data and results with an easy-read scientific report that summarizes the process, the generated data, and the results. To make the report fit into a standard scientific workflow we have structured the report, so it follows the structure of a traditional scientific publication. Also, all raw data is available for you to keep for any potential future use.

Advantages of 16S rRNA Sequencing Service

• Fast turn-around time
• Highly experienced personnel
• High-quality data quality
• Customized bioinformatics solutions

With a team of microbiologists from different scientific backgrounds, Creative Biolabs guides you from project inception to high-quality results. In the unit of Gut Microbiota Genomic, we provide comprehensive and professional services to meet all the needs of our customers. If you have interest, please contact us for further discussion about your microbiome project needs.

For Research Use Only | Not For Clinical Use