A549 In Vitro (3)H-thymidine Incorporation Assay (Cell Proliferation) (CAT#: ITS-1022-YF1692)

A549

Human

A549 cells were isolated from the lung tissue of a White, 58-year-old male with lung cancer. This cell line can be used in cancer, immuno-oncology and toxicology research.

Lung Carcinoma

Oncology

In Vitro (3)H-thymidine Incorporation Assay (Cell Proliferation)

A549-cell based In Vitro (3)H-thymidine Incorporation Assay (Cell Proliferation)

The 3H-thymidine incorporation assay is one such assay that can detect newly synthesized DNA in cells. Cultured cells are mixed with 3H-thymidine (radioactive) and kept for a desired incubation period. Newly replicated DNA strands can incorporate 3H-thymidine during mitosis. A scintillation counter is necessary to detect the output. Direct measurement of proliferating cells is the main advantage of this assay. However, use of harmful radioactive substances and need of special equipment are considered as main disadvantages.

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Proliferation Assay

Cell-based Assays Based on DNA Synthesis