ARH-77 In Vitro Lactate Assay (Angiogenesis) (CAT#: ITS-1122-YF4988)

ARH-77

Human

The ARH-77 cell line was established from the peripheral blood of a patient suffering from IgG plasma cell leukemia. Although established from cells taken from a patient with a plasma cell leukemia, this line has been shown to be an EBV-transformed B lymphoblastoid cell line.

Plasma Cell Leukemia

Oncology

In Vitro Lactate Assay (Angiogenesis)

ARH-77-cell based In Vitro Lactate Assay (Angiogenesis)

Accumulation of lactate in cancer cells has been linked with poor survival and cancer metastasis. Higher glucose uptake in cancer cells is considered as the main cause for the accumulation and secretion of lactate by cancer cells. The lactate/pyruvate ratio is an essential parameter for the maintenance of the redox state of cells. Therefore, evaluation of cellular lactate levels allows assessment of the cellular oxygen utilization and demand. A number of commercially available colorimetric or fluorometric kits have been developed to detect lactate levels in cells and tissues.

Angiogenesis Monitoring Assays

Local and regional metastasis is a life-threatening condition in cancer, which is hard to control with the available cancer treatment options. In metastasis, cancer cells move away from primary tumors and travel through the blood and lymphatic system and form new tumors on other parts. A vascular
network growth is important for cancer metastasis. Angiogenesis is a process that is known as the formation of new blood vessels with the help of existing blood vessels and angiogenesis has been reported to play a major role in cancer growth and metastasis. Tumors can send chemical signals to nearby normal tissues to stimulate angiogenesis. New blood vessels can feed growing tumors with nutrients and oxygen, allowing cancer cells to spread (metastasis).