B Cell-based Bio-layer Interferometry Assay
The Bio-layer interferometry (BLI) Technique
The bio-layer interferometry (BLI) biosensor system detects and analyzes the interference pattern of white light reflected from the plate where proteins are immobilized. In the BLI assay, the biomolecules in a sample can bind to the sensor plate and cause shifts in light reflection and interference patterns. Moreover, only protein binding or dissociation can change the interference pattern. Other parameters, such as refractive index, pH, and other matrix effects, do not influence the response profile. The changes are recorded in real-time and transferred to visual curves, enabling fast measurement of binding affinity, association, and dissociation kinetics of antibody-antigen interactions.
Fig.1 Schematics of bio-layer interferometry (BLI) strategy for the identification of active viral epitope.1
BLI Assay Services at Creative Biolabs
Creative Biolabs offers a label-free bio-layer interferometry assay that could quantitatively measure the kinetic characteristics of proteins for cancer epitope binning and is suitable for both academic and biopharmaceutical research.
Workflow
BLI Assay Arrangement and Data Process
The binding assay is conducted by exposing sensors with serially diluted antibody clones in the binding buffer in wells of a 96-well microtiter plate. Binding kinetics are measured using an advanced sensor and transformation system.
Fig.2 Representative curves and data from antigen-antibody binding and dissociation.2
Service Deliverables
The binding curves and binding kinetics data are obtained from sensorgrams.
Equilibrium Dissociation Constant (KD)
Key Features and Advantages of Our BLI Assay
BLI does not need labeling, avoiding much time spent on sample preparation and reducing binding signal influence from the label itself.
Dynamic interactions are captured and documented simultaneously, providing comprehensive data on specific interactions for decision-making.
We can run 96 samples with different concentrations in a single experiment to find the best binding conditions and can screen two thousand samples in about three-four hours, saving much time for results.
Online Consultation
With the advanced biolayer interferometry technology platform established for years and scientists experienced in cancer antigen discovery, Creative Biolabs provides incomparable BLI services to meet all your demands. We promise reliable, accurate, specific, reproducible, and high-quality BLI services for clients. Please contact us to get more information on the BLI assay.
References
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Petersen, R. L. Strategies Using Bio-Layer Interferometry Biosensor Technology for Vaccine Research and Development. Biosensors. 2017, 7(4): 49.
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Shah, N. B.; Duncan, T. M. Bio-layer interferometry for measuring kinetics of protein-protein interactions and allosteric ligand effects. Journal of visualized experiments : JoVE. 2014, (84): e51383.
For Research Use Only | Not For Clinical Use
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