COLO 205 In Vitro Protein Carbonylation Detection Assay (Oxidative Stress) (CAT#: ITS-1022-YF5632)

COLO 205

Human

The COLO 205 cell line is made up of epithelial cells isolated in 1975 from ascitic fluid derived from a 70-year-old, White, male with colon cancer. The cells can be used for cancer and toxicology research.

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro Protein Carbonylation Detection Assay (Oxidative Stress)

COLO 205-cell based In Vitro Protein Carbonylation Detection Assay (Oxidative Stress)

Assays for the detection of protein carbonylation have widely been used to determine oxidative stress. Protein carbonylation is one of the irreversible protein modification processes in oxidative stress, which includes modification of side chains of amino acids of proteins to carbonyl derivatives. A wide variety of assays and analytical techniques such as high-performance liquid chromatography (HPLC), gas chromatography, immunoassays, radioactive labeling and capillary electrophoresis have been used to detect protein carbonylation in oxidative stress.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Markers Thiobarbituric Acid Reactive Substances