Cow IL 18 ELISA Kit, Lot 21AO-1335 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P1246)

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Pre-coated

4 °C

6 months

For Research Use only

Interleukin 18 (IL-18)

Igif; Il-18; IL-18; IGIF; ChIL-18; IL-1g; IL1F4; IL18; interleukin 18; Il18; IL18

281249

Q9TU73

Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.