Engineered cancer cells can be a useful tool for clinical and therapeutic applications in cancer treatment. Creative Biolabs is committed to providing comprehensive and professional services for engineering Du-145 cells to meet your project needs.

Introduction of DU-145 Cell Line

DU-145 was the first prostate cancer cell line to be established in tissue culture. This cell line was isolated from a metastatic site in the brain of a 69-year-old, White, male with prostate cancer. The cells are epithelial morphology. It is an androgen-independent cell that has moderate metastatic potential but no endogenous androgen receptors (AR). These cells are not sensitive to hormones and fail to express prostate-specific antigen (PSA), but do express functional receptors activator of NF-kappa B and androgen receptors. Both PC3 and DU145 cells have been used extensively to model hormone-refractory and aggressive prostate cancer.

• Characteristics of DU-145 Cell Line

Engineering DU-145 Cell Service

• Strategies of Engineering DU-145 Cell

Cancer cell-based immunotherapy is a promising therapeutic strategy to combat a wide range of cancers. The inoculation of cytokine-engineered tumor cells can recruit and activate immune cells to produce antitumor effects. In addition, gene-modified cancer cells provide a large number and variety of antigens, that can be used as vaccines in the immunotherapy of cancer. At Creative Biolabs, we are dedicated to engineering cancer cells utilizing several strategies, thus turning cancer cells against themselves. Prostate cancer cell DU-145 can be designed to engineer with cytokines overexpression, as well as a target-specific aptamer, aptamer-siRNA, synthetic immunomodulatory gene circuits, etc.

• Technologies of Engineering DU-145 Cell

Our scientists are well-versed in working with different cellular backgrounds such as established cancer cell lines, primary cells, etc. Our scientists have extensive expertise and experience in gene delivery to different cells, which enables the construction of a variety of cell lines. A variety of cell lines are successfully engineered to achieve the desired attributes. Our expert team will deliver the custom-engineered DU-115 cells through several methods including lentivirus infection, retrovirus, CRISPR technology, etc.

• Characterization & Functional Assessment

Our capabilities also include a series of functional characterization involving self-destruction assays, immune reactivity assessment ex vivo, toxicity assessment, and circuit performance assessment. These services can further custom tune-up or optimization, and more.

• Workflow of A Typical Project

Creative Biolabs is one of the few providers of integrated upstream generation & downstream assessment services. We offer turn-key or a la carte services customized to our client’s needs. The following are services we can provide:

Our customer service representatives are available 24hr a day, 7 days a week. Contact us today for a free consultation with the scientific team and discover how Creative Biolabs can be a valuable resource and partner for your organization.

Reference

1. Brill, T.H.; et al. Allogeneic retrovirally transduced, IL-2-and IFN-γ-secreting cancer cell vaccine in patients with hormone refractory prostate cancer—a phase I clinical trial. The Journal of Gene Medicine: A cross‐disciplinary journal for research on the science of gene transfer and its clinical applications. 2007, 9(7): 547-560.

For Research Use Only | Not For Clinical Use