HeLa In Vitro WST Assay (Cell Viability) (CAT#: ITS-1022-YF5150)

HeLa

Human

In 1952, HeLa cells became the first human cell line that could grow and divide endlessly in a laboratory, leading scientists to label these cells "immortal". The immortality of HeLa cells contributed to their adoption across the world as the human cell line of choice for biomedical research.

Cervical Cancer

Oncology

In Vitro WST Assay (Cell Viability)

HeLa-cell based In Vitro WST Assay (Cell Viability)

Apart from the MTT tetrazolium, other tetrazolium compounds such as WST (reagent series) can also generate formazan upon reduction by oxidoreductase or dehydrogenase. Unlike the normal MTT assay, assays with these regents (MTS, XTT and WST) do not require formazan solubilization step as solubilization occurs in the cell medium itself. Therefore, XTT, WST and MTS reagents can be used to assay viability of cells, which do not require a surface to attach.

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Viability Assay

Cell-based Viability Assays Based on Cellular Enzymes and Proteins