Hep 3B In Vitro Mitotic Index Assay (Cell Proliferation) (CAT#: ITS-1022-YF2113)

Hep 3B

Human

Hep 3B is a cell line exhibiting epithelial morphology that was isolated from liver tissue derived from an 8-year-old, Black youth with liver cancer. This cell line contains an integrated hepatitis B virus genome.

Hep-3B; Hep3B; Hep 3B2.1-7

Hepatocellular Carcinoma

Oncology

In Vitro Mitotic Index Assay (Cell Proliferation)

Hep 3B-cell based In Vitro Mitotic Index Assay (Cell Proliferation)

Mitotic index assay is one of the old assays used to determine the number of cells that are undergoing mitosis in a specified cell population using monoclonal antibodies specific for phosphorylated histone H3 (ser28). Phosphorylated histone H3 (ser28) is considered as a main marker for mitosis and this specific phosphorylation can be utilized to calculate the mitotic index in a specified cell population.

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Proliferation Assay

Cell-based Assays Based on DNA Synthesis