High-Throughput Antibody Production Platform

HT Antibody Sequencing & Engineering

If an antibody with known sequence is available, we perform a sequence analysis to identify the variable regions (VH and VL) and the constant regions (CH and CL) of the antibody. If unknown, we perform HT sequencing service like:

• High-Throughput Hybridoma Sequencing
• High-Throughput de novo Monoclonal Antibody Sequencing
• High-Throughput Polyclonal Antibody Sequencing
• High-Throughput Single-cell BCR Sequencing (scBCR-seq)

HT Gene Synthesis & Vector Construction

We proceed gene synthesis and expression vectors with HT platform, including:

• High-Throughput Silicon-based DNA Synthesis Platform
• High-Throughput Vector Cloning Platform
• High-Throughput Plasmid Preparation Platform

HT Stable Transfection

We perform stable transfection to introduce the expression vectors into the host cells. The choice of expression system depends on the specific requirements of the project.

• High-Throughput DNA Plasmid Transfection Platform
• High-Throughput CHO Antibody Prodcution Platform
• High-Throughput HEK Antibody Prodcution Platform

HT Antibody Expression & Purification

Once the recombinant antibodies are produced, We use High-Throughput Antibody Purification Platform to obtain a highly pure and functional antibody preparation. The purification techniques include affinity chromatography, ion-exchange chromatography, size exclusion chromatography, and protein A/G purification.

HT Antibody Characterization

We conduct thorough characterization and quality control to ensure their quality and functionality. This includes:

• High-Throughput SPR Platform for Antibody Affinity Characterization
• High-Throughput ELISA Platform for Antibody Kinetics Characterization
• High-Throughput Mass Spectrometry Platform for Antibody Developability Characterization
• High-Throughput HPLC Platform for Antibody Developability Characterization