HL-60 In Vitro Cell Exclusion Zone Assay (Migration) (CAT#: ITS-1122-YF3705)

HL-60

Human

The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36-48 hours. The cell line was derived from a 36-year-old woman who was originally reported to have acute promyelocytic leukemia at the MD Anderson Cancer Center.

HL60

Leukemia

Oncology

In Vitro Cell Exclusion Zone Assay (Migration)

HL-60-cell based In Vitro Cell Exclusion Zone Assay (Migration)

For the cell exclusion assay, before seeding of cells in well plates, silicon stoppers (for 96- or 384-well formats) are placed inside the wells to create a cell free zone. Once cells attain confluency, stoppers are removed, allowing cells to cover the exclusion zone. Unlike in the normal scratch assay, sharp and uniform borders can be created with the stoppers. When placing a stopper inside wells, stopper must be firmly attached to the bottom of the well plate to prevent movement of cells beneath the stopper. Cells moving towards the exclusion area can be monitored using light or fluorescence microscopy.

Cell Migration and Invasion Assays

The movement of cancer cells and formation of tumors in neighboring tissue is a sequential cascade known as invasion-metastatic cascade, which includes cancer cell invasion through extracellular matrix (ECM), intravasation into blood and colonization at sites after they exit from blood circulation. Migration and invasion are two dif ferent processes where, cell migration involves movement of cells without passing through a barrier such as ECM, whereas cell invasion involves passing through a barrier such as ECM while destroying it.

Cell Migration Assay

Cell Migration Assay