LS 174T In Vitro Assessment of Glycolytic Enzymes Assay (Energy Metabolism) (CAT#: ITS-1122-YF365)

LS 174T

Human

LS 174T is a cell exhibiting epithelial morphology that was isolated from the colon of a White, 58-year-old, female adenocarcinoma patient with colorectal cancer. This cell line was deposited by Northwestern University and can be used in cancer research.

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro Assessment of Glycolytic Enzymes Assay (Energy Metabolism)

LS 174T-cell based In Vitro Assessment of Glycolytic Enzymes Assay (Energy Metabolism)

Glucose-6-phosphate dehydrogenase, pyruvate kinase, phosphofructokinase and hexokinase are the main enzymes involved in glycolysis. Among these, pyruvate kinase, phosphofructokinase and hexokinase are considered as rate-limiting enzymes. Conversion ofNAD+ to NADH- or NADP+ to NADPH has been combined with testing enzymes in many ready-made assay kits in which enzyme activity can be detected calorimetrically or fluorometrically.

Energy Metabolism Detection Assays

Cells require energy to grow, replicate and maintain internal cellular environment. Cells acquire energy need through a series of metabolic reactions known as respiration. Energy is stored as ATP in cells and when cells require energy, ATP can be broken down to fulfil the energy needs of cells. Aerobic and anaerobic respirations are two main forms of cellular respiration. Aerobic respiration requires oxygen to break down glucose into pyruvate, ATP and carbon dioxide and in anaerobic respiration, cells break glucose into lactic acid instead of pyruvate. Production of ATP is higher in the aerobic respiration (38 ATPs) than in anaerobic respiration. Glycolysis is the first step in cellular respiration, which involves production of pyruvate from glucose molecules. Enhanced rate of glycolysis is one of the main observations in cancer cells compared to normal cells, which is known as the Warburg effect. The consumption of glucose is reported to be higher in cancer cells than in normal cells. It has been reported that cancer cells can secrete lactic acid rather than converting glucose molecules into carbon dioxide. When considering the rate of ATP production in cancer cells, it has been reported that cancer cells can produce ATP about hundred-fold faster than normal cells. High expression of glucose receptors on the cell surface and enzymes controlling glucose influx into cancer cells have been identified as the main reasons for increased uptake of glucose by cancer cells. Measuring metabolites and enzymes in energy metabolism of cancer has been pivotal in medical research and a wide range of manual assay procedures and kits are available to measure changes in energy metabolism in cancer cells.

Assessment of Glycolytic Enzymes Assay