Assay Name
In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress)
Short Description
MCF7-cell based In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress)
Assay Description
It's a commonly used simple antioxidant assay. In the DPPH assays, DPPH free radical (DPPH) accepts protons from compounds with antioxidant properties to form yellow colored stable DPPH.
Assay Type
Oxidative Stress Assays
Assay Type Details
Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.
Assay Subtype
Antioxidant Assay