NCI-H1975 In Vitro Colony Formation Assay (Cell Proliferation) (CAT#: ITS-1022-YF2439)

NCI-H1975

Human

NCI-H1975 [H-1975, H1975] is a cell line exhibiting epithelial morphology that was isolated in 1988 from the lungs of a nonsmoking female with non-small cell lung cancer. Use these cells in immuno-oncology and lung cancer research.

H-1975; H1975

Lung Adenocarcinoma

Oncology

In Vitro Colony Formation Assay (Cell Proliferation)

NCI-H1975-cell based In Vitro Colony Formation Assay (Cell Proliferation)

Colony formation assay is one of the widely used assays based on the ability of cancer cells to form into colonies. This assay is useful to determine the effects of a drug or external stimuli such as radiation on clonogenic growth of cancer cells. This can be easily carried out for any type of cancer cells grown as a monolayer or as non-adherent cells. Before starting cell seeding, all the cells to be plated must be properly detached to avoid colonies at the starting point. A colony must contain a minimum of 50 cells and formed colonies are counted after staining with a suitable dye such as trypan blue. A microscope is usually used to visualize cell colonies and counting is normally carried out manually.

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Proliferation Assay

Colony Formation Assay