KCNH5 Membrane Protein Introduction

Introduction of KCNH5

KCNH5, also known as potassium voltage-gated channel, subfamily H (eag-related), member 5, is encoded by KCNH5 gene. It has the evolutionary conservative function of promoting the growth and metastasis of brain tumor, depicts the downstream pathway, and reveals the functional cooperation of different potassium channels and the mechanism of regulating the volume of mitotic cells and tumor progression. Meanwhile, some studies have identified that only in the human placenta, the expression of voltage-gated potassium channels gene KCNH5 transposon-derived transcript after low methylation, which indicates KCNH5 could be a biomarker in the clinic.

Basic Information of KCNH5
Protein Name	Potassium voltage-gated channel subfamily H member 5
Gene Name	KCNH5
Aliases	EAG2, H-EAG2, Kv10.2, hEAG2
Organism	Homo sapiens (Human)
UniProt ID	Q8NCM2
Transmembrane Times	7
Length (aa)	988
Sequence	MPGGKRGLVAPQNTFLENIVRRSSESSFLLGNAQIVDWPVVYSNDGFCKLSGYHRADVMQKSSTCSFMYGELTDKKTIEKVRQTFDNYESNCFEVLLYKKNRTPVWFYMQIAPIRNEHEKVVLFLCTFKDITLFKQPIEDDSTKGWTKFARLTRALTNSRSVLQQLTPMNKTEVVHKHSRLAEVLQLGSDILPQYKQEAPKTPPHIILHYCAFKTTWDWVILILTFYTAIMVPYNVSFKTKQNNIAWLVLDSVVDVIFLVDIVLNFHTTFVGPGGEVISDPKLIRMNYLKTWFVIDLLSCLPYDIINAFENVDEGISSLFSSLKVVRLLRLGRVARKLDHYLEYGAAVLVLLVCVFGLVAHWLACIWYSIGDYEVIDEVTNTIQIDSWLYQLALSIGTPYRYNTSAGIWEGGPSKDSLYVSSLYFTMTSLTTIGFGNIAPTTDVEKMFSVAMMMVGSLLYATIFGNVTTIFQQMYANTNRYHEMLNNVRDFLKLYQVPKGLSERVMDYIVSTWSMSKGIDTEKVLSICPKDMRADICVHLNRKVFNEHPAFRLASDGCLRALAVEFQTIHCAPGDLIYHAGESVDALCFVVSGSLEVIQDDEVVAILGKGDVFGDIFWKETTLAHACANVRALTYCDLHIIKREALLKVLDFYTAFANSFSRNLTLTCNLRKRIIFRKISDVKKEEEERLRQKNEVTLSIPVDHPVRKLFQKFKQQKELRNQGSTQGDPERNQLQVESRSLQNGASITGTSVVTVSQITPIQTSLAYVKTSESLKQNNRDAMELKPNGGADQKCLKVNSPIRMKNGNGKGWLRLKNNMGAHEEKKEDWNNVTKAESMGLLSEDPKSSDSENSVTKNPLRKTDSCDSGITKSDLRLDKAGEARSPLEHSPIQADAKHPFYPIPEQALQTTLQEVKHELKEDIQLLSCRMTALEKQVAEILKILSEKSVPQASSPKSQMPLQVPPQIPCQDIFSVSRPESPESDKDEIHF

Function of KCNH5 Membrane Protein

KCNH5 is abundant in the brain and enhances cancer cell growth by controlling cell volume. The channel contains a cyclic nucleotide-binding homology (CNBH) domain and multiple calmodulin-binding motifs. KCNH5 plays a vital role in many biological functions, such as epithelial electrolyte transport, cell cycle, and proliferation. Previous studies have shown that it has high expression in several somatic cancer cell lines (including melanoma), normal adult brain and placenta, which suggest it could be involved in cell cycle regulation and tumor progression in cancer. In addition, studies have confirmed that KCNH5 is associated with epilepsy.

Fig.1 Structure of voltage-gated potassium channel.

Application of KCNH5 Membrane Protein in Literature

Niday Z., et al. Potassium Channel Gain of Function in Epilepsy: An Unresolved Paradox. Neuroscientist. 2018, 24(4):368-380. PubMed ID: 29542386

This article analyses the expression levels of these epilepsy-related genes (KCNA2, KCNB1, KCND2, KCNH1, KCNH5, KCNJ10, KCNMA1, KCNQ2, KCNQ3, and KCNT1) and identifies the potential mechanisms of the development of seizures and epilepsy in patients. These results show that these genes may be a target for clinical therapy.
Huang X., et al. EAG2 potassium channel with evolutionarily conserved function as a brain tumor target. Nat Neurosci. 2015, 18(9): 1236-46. PubMed ID: 26258683

This article reveals that antipsychotic drug thioridazine as an EAG2 channel blocker that reduces xenografted MB growth and metastasis and presents a case report of repurposing thioridazine for treating a human patient.
Shimizu N., et al. A sustained increase in the intracellular Ca²⁺ concentration induces proteolytic cleavage of EAG2 channel. Int J Biochem Cell Biol. 2015, 59: 126-34. PubMed ID: 25542181

Authors in this group focus on the pharmacological activation of EAG2 channel. The data indicate that Ca²⁺ concentration may play an important role in activating the EAG2 channel when under physiological and/or pathological conditions.
Joe I.S., et al. PDE4 Inhibition by Rolipram Promotes Neuronal Differentiation in Human Bone Marrow Mesenchymal Stem Cells. Cell Reprogram. 2016, 18(4): 224-9. PubMed ID: 27459581

This article examines the expression levels of neuronal-specific marker genes (CD133, GFAP, NF-M, MAP-2, KCNH1, KCNH5, SCN3A, and CACNA1A) in human bone marrow mesenchymal stem cells. The results suggest a higher expression of these genes may be related to the development of neurodegenerative disorders.
Macaulay E.C., et al. Retrotransposon hypomethylation in melanoma and expression of a placenta-specific gene. PLoS One. 2014, 9(4): e95840. PubMed ID: 24759919

This article evaluates the methylate and expression of the reverse transcription protein KCNH5 in 25 melanoma cell line to determine whether the apparent genetic marker of the "placenta" is related to melanoma.

KCNH5 Preparation Options

To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-KCNH5 antibody development services.

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