DBCO-Liposome (PEGylated) (CAT#: CLBD035LY)

Description
DBCO-Liposome (PEGylated) is Liposome (PEGylated) with the dibenzocyclooctyne (DBCO) group, which can react with an azide by click chemistry. The conjugation chemistry is based on the reaction of the DBCO reagent with an azide linker to form a stable triazole. Azide moiety can be on the antibody and DBCO moiety can be on liposomes and vice versa.
Lipid Composition
Hydrogenated Soy PC, Cholesterol, DSPE-PEG(2000), DSPE-PEG(2000)-DBCO
Buffer
PBS
pH
7.4
Storage
Store in dark at +4°C and do not freeze
Size
100 nm
Quantity
2, 5 mL
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DataSheet MSDS
FAQs Published Data Customer Reviews Related Sections
  1. What is DBCO-Liposome (PEGylated)?

    DBCO-Liposome (PEGylated) is a liposome conjugated with a dibenzocyclooctyne (DBCO) group.

  2. What is the main purpose of using DBCO-Liposome (PEGylated)?

    It is used for targeted delivery, facilitating the attachment of various ligands, such as antibodies or peptides, to liposomes.

  3. Is the PEGylation of liposomes important?

    PEGylation increases liposome circulation time in the body, enhances biocompatibility, and reduces immunogenicity.

  4. What role does cholesterol play in the liposome structure?

    Cholesterol enhances the liposome's membrane fluidity and stability.

DBCO-Liposome (PEGylated)-fig1


Confocal fluorescence microscopy images showing the influence of different MSNP coatings on the uptake of nanoparticles in A549 and HepG2 cells.

This study focuses on the conjugation of antibodies with nanoparticles to enhance targeting efficiency. The researchers functionalized the antibodies with dibenzylcyclooctyne (DBCO) while attaching azide (N3) groups to the nanoparticles (N₃-PEI-MSNPs). Subsequently, the antibody was covalently linked to the nanoparticles through copper-free click chemistry between the azide moiety and the DBCO-labeled antibody, resulting in αCD44 PEI-MSNPs. The targeting results demonstrated that αCD44 PEI-MSNPs can selectively target A549 cells with high CD44 expression. This research highlights the critical role of DBCO in the conjugation of antibodies and nanoparticles, showcasing significant potential for improving the targeting efficiency of nanoparticles.


Van Zundert, Indra, et al. "Versatile and Robust Method for Antibody Conjugation to Nanoparticles with High Targeting Efficiency." Pharmaceutics 13.12 (2021): 2153.

Distributed under Open Access license CC BY 4.0, without modification.

  • Targeted Delivery
    These liposomes have been instrumental in my research on the targeted delivery of therapeutic peptides.
  • Successful Conjugation
    The click chemistry with these liposomes is incredibly efficient, making the conjugation process a breeze.
  • Excellent Circulation Time
    Thanks to PEGylation, our liposomes exhibit extended circulation times, improving the overall efficacy of our drug delivery systems.
  • Robust Liposome Structure
    The inclusion of hydrogenated soy PC and cholesterol gives the liposomes exceptional structural stability, crucial for our robust research needs.
  • Optimal for Personalization
    Creative Biolabs' DBCO Liposome (PEGylated) allows us to personalize our liposomes for different targeting molecules, which is a game-changer for our tailored therapies.

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For Research Use Only. Not For Clinical Use

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