DOPG liposomes are composed mainly of dioleoylphosphatidylglycerol (DOPG), which is a phospholipid known for its negative charge at physiological pH. This property makes DOPG liposomes particularly useful in research involving negatively charged molecules or environments.
Due to their negative charge, DOPG liposomes are commonly used in the study of interaction with positively charged molecules, such as cationic proteins or peptides, and can be crucial in the study of cellular uptake mechanisms, drug delivery systems, and immune response evaluations.
The negative charge of DOPG liposomes can be utilized to encapsulate and deliver charged therapeutic agents more effectively. This feature is particularly advantageous in targeted drug delivery strategies, improving the encapsulation stability and distribution of drugs to specific sites within the body.
Yes, DOPG liposomes can be engineered to encapsulate genetic material such as DNA or RNA. Their ability to form complexes with nucleic acids makes them suitable for applications in gene delivery, potentially enhancing the efficiency of gene transfer techniques.
Techniques such as dynamic light scattering (DLS) for size measurement and zeta potential analysis for surface charge characterization are commonly used. These methods provide essential information about the stability and potential cellular interaction of DOPG liposomes.
DOPG increased cholesterol efflux from BMdM with U18666A decreasing efflux
The research investigates how DOPG liposomes modulate lysosomal cholesterol and its impact on silica-induced lysosomal membrane permeability (LMP) and inflammation. Inhalation of crystalline silica is known to cause lung diseases by damaging lysosomal membranes in macrophages. The study uses murine bone marrow-derived macrophages (BMdM) to examine how reducing lysosomal cholesterol with DOPG liposome treatment affects silica-induced LMP and IL-1β release. Results indicate that DOPG treatment enhances silica-induced LMP and IL-1β release. Specifically, BMdM treated with DOPG liposomes showed increased IL-1β release (measured by ELISA) and higher cell death rates (measured by LDH release assay) compared to control groups. Additionally, DOPG increased cholesterol efflux from lysosomes. DOPG-treated cells had a significant increase in cholesterol efflux, demonstrating its role in reducing lysosomal cholesterol content. These findings highlight the significance of lysosomal cholesterol manipulation in mitigating silica-induced lysosomal damage and inflammation, providing insights into potential therapeutic strategies for silica-related lung diseases.
Sydor, M. J., Kendall, R. L., & Holian, A. Cholesterol content regulates silica-induced lysosomal membrane permeability. Frontiers in Toxicology. 2023, 5: 1112822. Under Open Access license CC BY 4.0, without modification.
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