DOPG Liposomes (CAT#: LDLY-0123-LY28)

Description
Phosphatidylserine (PS) and phosphatidylglycerol (PG) are anionic molecules that form liposomes with negative zeta potential. The zeta potential of the liposomes depending on the molar percentage of the negatively charged lipids in the liposome formulation.
Lipid Composition
DOPG
Applications
Control liposomes, Membrane biology
Format
Liquid
Concentration
10mg/mL
Hydration Solution
PBS, pH 7.4
External Solution
PBS, pH 7.4
Storage
2°C - 8°C (do not freeze)
Size
100 nm
Shelf Life
6 months
Quantity
5mL (available in lyophilized powder)
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DataSheet MSDS
FAQs Published Data Customer Reviews Related Sections
  1. What is the primary composition of DOPG liposomes?

    DOPG liposomes are composed mainly of dioleoylphosphatidylglycerol (DOPG), which is a phospholipid known for its negative charge at physiological pH. This property makes DOPG liposomes particularly useful in research involving negatively charged molecules or environments.

  2. What are the typical applications of DOPG liposomes in biomedical research?

    Due to their negative charge, DOPG liposomes are commonly used in the study of interaction with positively charged molecules, such as cationic proteins or peptides, and can be crucial in the study of cellular uptake mechanisms, drug delivery systems, and immune response evaluations.

  3. How do DOPG liposomes enhance drug delivery systems?

    The negative charge of DOPG liposomes can be utilized to encapsulate and deliver charged therapeutic agents more effectively. This feature is particularly advantageous in targeted drug delivery strategies, improving the encapsulation stability and distribution of drugs to specific sites within the body.

  4. Can DOPG liposomes be used in gene therapy?

    Yes, DOPG liposomes can be engineered to encapsulate genetic material such as DNA or RNA. Their ability to form complexes with nucleic acids makes them suitable for applications in gene delivery, potentially enhancing the efficiency of gene transfer techniques.

  5. What methods are used to measure the effectiveness of DOPG liposomes in drug delivery?

    Techniques such as dynamic light scattering (DLS) for size measurement and zeta potential analysis for surface charge characterization are commonly used. These methods provide essential information about the stability and potential cellular interaction of DOPG liposomes.

DOPG Liposomes-fig1


DOPG increased cholesterol efflux from BMdM with U18666A decreasing efflux

The research investigates how DOPG liposomes modulate lysosomal cholesterol and its impact on silica-induced lysosomal membrane permeability (LMP) and inflammation. Inhalation of crystalline silica is known to cause lung diseases by damaging lysosomal membranes in macrophages. The study uses murine bone marrow-derived macrophages (BMdM) to examine how reducing lysosomal cholesterol with DOPG liposome treatment affects silica-induced LMP and IL-1β release. Results indicate that DOPG treatment enhances silica-induced LMP and IL-1β release. Specifically, BMdM treated with DOPG liposomes showed increased IL-1β release (measured by ELISA) and higher cell death rates (measured by LDH release assay) compared to control groups. Additionally, DOPG increased cholesterol efflux from lysosomes. DOPG-treated cells had a significant increase in cholesterol efflux, demonstrating its role in reducing lysosomal cholesterol content. These findings highlight the significance of lysosomal cholesterol manipulation in mitigating silica-induced lysosomal damage and inflammation, providing insights into potential therapeutic strategies for silica-related lung diseases.

Sydor, M. J., Kendall, R. L., & Holian, A. Cholesterol content regulates silica-induced lysosomal membrane permeability. Frontiers in Toxicology. 2023, 5: 1112822. Under Open Access license CC BY 4.0, without modification.

  • Superior Anionic Properties
    Creative Biolabs' DOPG liposomes exhibit superior anionic properties, enhancing the interaction with cationic drug molecules for more effective delivery.
  • Ideal for Sensitive Compounds
    The DOPG liposomes provide an ideal environment for sensitive compounds, ensuring stability and protection throughout the delivery process.
  • Customizable for Targeted Delivery
    Creative Biolabs offers DOPG liposomes that can be customized for targeted delivery, meeting specific research requirements effectively.
  • Reliable in Various pH Conditions
    The stability of DOPG liposomes in various pH conditions makes them reliable carriers for drug delivery across different biological environments.
  • Effective in Gene Delivery Applications
    Creative Biolabs' DOPG liposomes are particularly effective in gene delivery applications, facilitating efficient and safe transfer of genetic material.

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