As a pioneer and undisputed global leader in immune repertoire analysis, Creative Biolabs has developed a Magic™ platform for large-scale sequencing service. Our scientists have employed a powerful strategy to identify the vast majority of BCR V(D)JC sequences of B-cell receptor (BCR) repertoire in canine. We are more than happy to share our experience in canine BCR repertoire analysis to facilitate their meaningful immune repertoire projects.

Canine BCR Repertoire Properties

Complementarity determining regions (CDRs) are responsible for binding antigen and provide substantial diversity to the BCR repertoire. V_H CDR3 of the immunoglobulin variable heavy (V_H) domain plays a dominant role in immune response. The canine repertoire is heavily dominated by IGHV1 subgroup, which is evolutionarily close to the human IGHV3 subgroup. The productive canine repertoire shows many similarities to both human and mouse. However, there are distinct differences in terms of V_H CDR3 length and amino acid paratope composition. Compared to human and murine antibody repertoire, the length of canine V_H CDR3 regions is longer than the murine V_H CDR3 but shorter than the human counterparts. The amino acids at the base of the V_H CDR3 loop are strictly conserved, which is similar to corresponding human and mouse V_H CDR3. Moreover, there are significant changes in chemical paratope composition in CDR positions. Furthermore, canine V_H CDR3 displays an overrepresentation of the neutral amino acid threonine and the negatively charged aspartic acid while proline content is similar to that in the human repertoire. Overall the canine repertoire shows a bias towards small, negatively charged amino acids.

Fig.1 CDR3 lengths for the heavy (A) and light chains (B) of the healthy dogs.¹

Canine BCR Repertoire Profiling in Creative Biolabs

Through our powerful Magic™ platform, we can provide one-stop service including high-throughput sequencing and data analysis. PBMC, bone marrow or spleen, or RNA from them can be good starting materials as the template for the amplification of CDR. Both the heavy chain and the light chain CDR 3 regions of canine can be 100% covered and accurately sequenced. We can offer our customers comprehensive BCR repertoire analysis, including hypermutation and isotype data.

Key Advantages of Canine BCR Repertoire Analysis Service

• BCR repertoire profiling in canine
• PBMC, bone marrow or spleen, or RNA from them as starting materials
• Low input
• Highly professional Ph.D. level scientists
• Magic™ BCR repertoire analysis platform

Creative Biolabs is one of the well-recognized experts and we are professional in BCR repertoire analysis in canine. With years of experience, our scientists can offer high-quality service of canine BCR repertoire analysis. We are pleased to use our extensive experience and advanced platform to offer the best service of BCR repertoire profiling in canine to satisfy each demand from our customers.

Please contact us for more information and a detailed quote.

Diverse Species of Our Unbiased BCR Repertoire Profiling

• Feline Immunoglobulin Repertoire Profiling
• Zebrafish Antibody Repertoire Analysis
• Alpaca BCR Repertoire Profiling
• Rabbit BCR Repertoire Profiling
• Swine BCR Repertoire Profiling

Applications of Our Unbiased BCR Repertoire Profiling

• B Cell Clones Identification
• Novel Alleles Identification
• SHM Patterns Analysis

FAQ

1. What is BCR repertoire analysis in canines

   BCR (B cell receptor) repertoire analysis in canines refers to studying the diversity and clonality of B cell receptors in dogs. This involves sequencing BCRs to understand the range of immune responses. It is particularly useful for investigating canine immunological diseases, vaccine responses, and the overall functioning of the canine immune system.

2. Why is BCR repertoire analysis important in canines

   BCR repertoire analysis in canines provides valuable insights into their immune response to diseases and infections. By studying the diversity of B cell receptors, researchers can understand how the immune system combats pathogens and how B cells contribute to both protective immunity and autoimmune conditions. It is also useful for developing treatments for canine diseases.

3. What technologies are used for canine BCR repertoire analysis

   Next-generation sequencing (NGS) is the primary technology used in canine BCR repertoire analysis. This allows for high-throughput sequencing of the B cell receptor variable regions, providing a detailed view of the immune response. Bioinformatics tools are then employed to analyze the sequence data, revealing patterns of immune diversity and clonal expansion in canines.

4. How does BCR repertoire analysis contribute to canine immunotherapy research

   BCR repertoire analysis is essential in studying the immune landscape of canines in the context of immunotherapy. By profiling B cell receptors in dogs with cancers or chronic infections, researchers can identify key B cell populations involved in immune responses. This data helps design more effective immunotherapies tailored to the canine immune system, improving outcomes for diseases such as cancer.

5. Can BCR repertoire analysis in canines be used for comparative immunology studies

   BCR repertoire analysis in canines provides a model for comparative immunology. Since dogs share many diseases with humans, including cancers and autoimmune disorders, analyzing the canine immune system can provide insights into human immunology. Understanding similarities and differences between species enhances knowledge of immune system evolution and aids in the development of cross-species treatments.

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