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Magic™ Cell-free Membrane Protein Expression in Mammalian Cell Lysate (CAT#: MPCFK-2)

Datasheet
Magic™ Cell-free Protein Expression in Mammalian Cell Lysate Kit is a cell-free protein synthesis system using human cell extracts. This system contains all the required components for protein synthesis (ribosomes, translation initiation /elongation /enhancement factors, tRNA, polymerase, amino acids, ATP etc.) and allows protein synthesis to occur at high efficiency and high yield. Modifications such as glycosylation and phosphorylation could be easily obtained. The procedure can be performed in a single reaction tube from RNA transcription to protein synthesis.
The transcribed target gene RNA contains an IRES sequence designed to promote protein translation initiation. Although the translation initiation factor in the cell extract becomes inactivated as protein synthesis progresses, it can be reactivated by the translation enhancement factor included in the Accessory Proteins, therefore maintaining the translation level.

Product Specifications

  • Lysate Type
  • Mammalian Cell Lysate
  • Size
  • 10 reactions; 20 reactions
  • Reaction Volume
  • 20 µL per reaction
  • Expected Yield of Positive Control
  • 2.5 mg/ml
  • Components
  • Human Cell Extract
    Reaction Buffer
    Accessory Proteins
    Amino Acid Mix
    Cloning Vector
    T7 RNA Polymerase
    Control Vector
  • Storage Conditions
  • Store at -80°C.

Customer reviews and Q&As    

Customer Review

Glycosylated proteins can be obtained

This method can easily obtain glycosylated proteins.
2023.1.05

Q&As

How to reduce cell-free expression in double emulsion-coated mammals?

Related studies show that protein aggregation by polyvinyl alcohol surfactant reduces cell-free expression in double emulsion-coated mammals.
2023.01.08

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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