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Magic™ Cell-free Membrane Protein Expression in Rabbit Reticulocyte Lysate (CAT#: MPCFK-6)

Datasheet
Magic™ Cell-free Protein Expression in Rabbit Reticulocyte kit is a cell-free protein synthesis system using rabbit retic lysate and is optimized for a variety of high specific activity protein synthesis from capped and uncapped mRNA and for appropriated protein folding. The kit contains the required components for protein synthesis. The optimized Reticulocyte Lysate in kit provides ATP-regeneration system, hemin and calf liver tRNA. The optimized Translation Mix (-Met or -Leu) for the translation of both capped and uncapped messages along with High and Low Salt buffers. Which allows protein synthesis to occur at high efficiency, protein yield, and biological activity.

Product Specifications

  • Lysate Type
  • Rabbit Reticulocyte Lysate
  • Size
  • 60 reactions
  • Reaction Volume
  • 25 µL per reaction
  • Expected Yield of Positive Control
  • The purer the RNA, the higher the yield of protein will be
  • Components
  • Retic Lysate
    20X Translation Mix minus-Met
    20X Translation Mix minus-Leu
    High Salt Mix minus-Met
    Low Salt Mix minus-Met
    Capped Xen-1α RNA (50 μg/mL)
    Nuclease-Free Water
  • Storage Conditions
  • Store at -80°C.

Customer reviews and Q&As    

Customer Review

The system is flexible

This kit improves the yield of cell-free membrane protein expression based on rabbit reticulocytes
2023.1.31

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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