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Magic™ Membrane Protein Human MPL (MPL proto-oncogene, thrombopoietin receptor) Expressed in CHO for Antibody Discovery, Partial (25-491aa) (CAT#: MPX0301K)

Datasheet
This product is a 53.4 kDa Human MPL membrane protein expressed in CHO. The protein is for research use only and is not approved for use in humans or in clinical diagnosis.

Product Specifications

  • Host Species
  • Human
  • Target Protein
  • MPL
  • Protein Length
  • Partial (25-491aa)
  • Protein Class
  • Receptor
  • Molecular Weight
  • 53.4 kDa
  • TMD
  • 1
  • Sequence
  • SQDVSLLASDSEPLKCFSRTFEDLTC
    FWDEEEAAPSGTYQLLYAYPREKPRACPLSSQSMPHFGTRYVCQFPDQEE
    VRLFFPLHLWVKNVFLNQTRTQRVLFVDSVGLPAPPSIIKAMGGSQPGEL
    QISWEEPAPEISDFLRYELRYGPRDPKNSTGPTVIQLIATETCCPALQRP
    HSASALDQSPCAQPTMPWQDGPKQTSPSREASALTAEGGSCLISGLQPGN
    SYWLQLRSEPDGISLGGSWGSWSLPVTVDLPGDAVALGLQCFTLDLKNVT
    CQWQQQDHASSQGFFYHSRARCCPRDRYPIWENCEEEEKTNPGLQTPQFS
    RCHFKSRNDSIIHILVEVTTAPGTVHSYLGSPFWIHQAVRLPTPNLHWRE
    ISSGHLELEWQHPSSWAAQETCYQLRYTGEGHQDWKVLEPPLGARGGTLE
    LRPRSRYRLQLRARLNGPTYQGPWSSWSDPTRVETATETAW

Product Description

  • Expression Systems
  • CHO
  • Tag
  • 6xHis tag at the C-terminus
  • Protein Format
  • Soluble
  • Reconstitution
  • Reconstitute at 250 μg/mL in sterile PBS.
  • Endotoxin
  • <0.10 EU per 1 μg of the protein by the LAL method.
  • Purity
  • >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Buffer
  • Lyophilized from a 0.2 μm filtered solution in PBS.

Target

  • Target Protein
  • MPL
  • Full Name
  • MPL proto-oncogene, thrombopoietin receptor
  • Introduction
  • In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
  • Alternative Names
  • MPL; MPLV; TPOR; C-MPL; CD110; THPOR; THCYT2; thrombopoietin receptor; TPO-R; myeloproliferative leukemia protein; myeloproliferative leukemia virus oncogene; proto-oncogene c-Mpl; MPL proto-oncogene, thrombopoietin receptor

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