Creative Biolabs offers the unparalleled mass sequencing service to analyze the B cell receptors (BCR) repertoires. Our scientists can provide Magic™ single-cell BCR profiling by high throughput sequencing single B cell receptors. Above all, our method can keep the original linkage between the paired heavy and light chains intact. This technique increases the efficiency of identifying disease biomarkers and designing therapeutic antibodies.
B cell receptors help the immune system detect antigens and trigger antibody production. Every individual harbors a repertoire of BCR specific to a range of antigens to which the person has been previously exposed. Each BCR consists of a variable heavy (VH) and a variable light (VL) chain. Original paired VH:VL chains are important because they can be used as markers for antibodies. These are useful for applications such as tracking B cells, monitoring for minimal residual disease and diagnostics. The paired sequences have the clinical potential of rapidly improving vaccine design and identifying neutralizing antibodies. High-throughput next-generation sequencing of B cell receptor repertoires could provide important information, which is the key to understanding the adaptive immune responses and for diagnostic and therapeutic applications. Most high-throughput sequencing methods can identify large numbers of VH and VL, but they need large quantities of B cell DNA as starting material. Therefore, information about the endogenous pairing of heavy and light chains is lost after bulk lysis of B cell populations. Existing single cell-based sequencing approaches, such as scRT-PCR and Sanger sequencing, could avoid this problem, but they typically require complex instrumentation, and the process is complicated, expensive and time-consuming.
Creative Biolabs has designed a new single-cell sequencing approach that would retain the original VH:VL pairing with high throughput using low reagent and lowering cost. Overview of the high-throughput method for paired VH:VL single B cell receptor analysis is shown (Fig.1). Briefly, B-cell populations are sorted. Single cells are isolated and equilibrated with lysis buffer. Then mRNA is captured on magnetic beads, RT-PCR by emulsion VH:VL linkage PCR. Next-generation sequencing is performed on our Magic™ platform. Bioinformatic processing is used to analyze the paired VH:VL repertoire. A huge advantage for our approach is that the assay does not have to be done with live cells at the sites of the early steps. This approach for sequencing the paired VH:VL repertoire enables rapid interrogation of the immune response and can be applied to investigate, vaccine efficacy, immune system health and autoimmunity in clinical.
The high-throughput identification of paired VH:VL also enables rapid generation of novel diagnostic, therapeutic or prophylactic antibodies. Scientists of Creative Biolabs have accumulated enough experience in high-throughput sequencing single-cell BCR with paired VH:VL. We are confident in providing clients with the best service at the most competitive cost.
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Learn more about Magic™ single-cell BCR profiling services:
Fig. 1 Dynamics of antiviral B cell response at single-cell resolution is organ specific.1
The study provides significant insights into how B cell responses develop and differentiate following respiratory viral infections. By combining single-cell RNA sequencing (scRNA-seq) with single-cell B cell receptor sequencing (scBCR-seq), the study maps the transcriptional profiles and clonal dynamics of B cells in different organs post-influenza infection. The findings highlight the differences in B cell memory formation and germinal center (GC) responses between organs like the lungs, spleen, and lymph nodes. Single-cell BCR profiling was crucial in identifying B cell clones and tracking their mutations, expansion, and affinity maturation across different organs and stages of the immune response. This service is essential for understanding clonal selection and immune memory development, providing valuable information for vaccine development and improving antiviral immunity strategies.
Single-cell BCR profiling is a method that uses high-throughput sequencing to capture the unique B cell receptor (BCR) sequences from individual B cells. This allows for the analysis of B cell diversity, clonal expansion, and immune responses at a single-cell level, providing detailed insights into how B cells function in health and disease.
Unlike bulk BCR sequencing, which captures the average BCR profile of a population of B cells, single-cell BCR profiling examines the receptors at the level of individual cells. This provides precise information about B cell clones, their diversity, and the transcriptional states of individual B cells, enabling a deeper understanding of immune responses.
Single-cell BCR profiling is used to study immune responses to infections, cancers, autoimmune diseases, and vaccines. By analyzing the clonal diversity of B cells at the single-cell level, researchers can identify antigen-specific B cells, track their evolution, and understand how B cell populations expand or mutate during immune responses.
High-throughput sequencing enables the rapid and comprehensive capture of BCR sequences from thousands of individual cells simultaneously. This allows researchers to study the diversity and clonality of B cell populations in great detail, helping to uncover how B cells contribute to immune responses, disease progression, or therapeutic outcomes.
Challenges in single-cell BCR profiling include the complexity of isolating and sequencing individual B cells, the need for high-quality sequencing data, and advanced bioinformatics tools to analyze the vast amount of data generated. Despite these challenges, technological advancements have made it easier to achieve accurate and comprehensive BCR profiling at the single-cell level.
Single-cell BCR profiling allows for a precise understanding of the immune repertoire, helping to identify B cell clones involved in disease or therapeutic responses. This enables personalized treatment strategies, such as designing therapies based on a patient's unique B cell profile. It also accelerates the development of immunotherapies, vaccines, and diagnostic tools by providing detailed insights into B cell function and diversity.
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All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
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