Repertoire Analysis Services

Background Service Platform Features Published Data FAQ

Introduction of Repertoire Analysis

Repertoire analysis utilizes high-throughput sequencing and bioinformatics to study the diversity and specificity of B cell receptors (BCR), T cell receptors (TCR), and antibodies. This analytical method provides detailed insights into how the immune system responds to pathogens and other foreign substances, helping to identify the best weapons against diseases.

Repertoire analysis not only aids scientists in developing new drugs but also enhances the accuracy of disease diagnosis, advancing medical technology. By combining phage display with repertoire analysis, Creative Biolabs can more efficiently discover and validate high-affinity and high-specificity candidate molecules, significantly promoting the progress of medical research and clinical applications.

Repertoire Analysis Services

Magic™ BCR Repertoire Analysis

BCR Repertoire Analysis

Comprehensive BCR repertoire from the RNA of either humans or mice.
Specific capture primers.
High fidelity amplification method.
Advanced high-throughput sequencing platform.

Magic™ TCR Repertoire Analysis

TCR Repertoire Analysis

Comprehensive TCR repertoire from the RNA of either humans or mice.
Specific capture primers.
High fidelity amplification method.
Advanced high-throughput sequencing platform.

Magic™ Peptide Repertoire Analysis

Peptide Repertoire Analysis

Combined with the phage display platform.
Custom peptide binder selection and epitope mapping.
Optimized sequencing analysis method.

Magic™ Antibody Repertoire Analysis

Antibody Repertoire Analysis

Integration of next generation sequencing (NGS) and bioinformatics in parallel.
Sequencing of diverse antibody repertoires at a rate of millions of clones per hour.
High-fidelity analysis of the full-length paired heavy- and light-chains expressed by individual B cells.
Analysis of Fab repertoire, scFv repertoire, and sdAb repertoire

Magic™ Bioactive Peptide Discovery

Bioactive Peptide Discovery

High-throughput.
High affinity.
Up to 300 unique binders.
Fast result.

Magic™ Quantitative Immune Repertoire for Small Lymphocytes

Quantitative Immune Repertoire for Small Lymphocytes

Qualitative and quantitative BCR/TCR proﬁling.
Special for small lymphocyte populations, such as Ag-speciﬁc, tissue-resident cell subsets, microdissection, or ﬁne needle aspirates.
Based on UMIs.
Low amount of sample input.

Related Tech Platform

Phage display platform is a powerful technology used to generate and screen large libraries of peptides or antibodies. It plays a crucial role in repertoire analysis by providing a diverse set of sequences for further study. The integration of the phage display platform with repertoire analysis provides a robust method for discovering and characterizing high-quality candidate molecules, thereby accelerating advancements in medical research and clinical applications.

Advantages

Comprehensive Diversity Analysis
High-Throughput Sequencing
Enhanced Specificity & Affinity
Advanced Bioinformatics
Accelerated Drug Development
Versatile Applications
Customizable Solutions

Published Data

Analysis of IgM and IgT Repertoires from Takifugu rubripes

Fig. 1 A figure presents the repertoire analysis results of three torafugu. (Fu, et al., 2018) Fig. 1 Overall IgM (A) and IgT (B) repertoires across the three torafugu.¹

Scientists analyzed the antibody library of torafugu and obtained 1.2 billion expressed antibody sequences from three healthy adults. The findings revealed convergence and evolution of CDR-H3 sequences in IgM and IgT, and a bias towards specific VDJ combinations in individual fish. Different components of IgM and IgT were also observed, suggesting varied mechanisms for diversity creation in the naïve immune system of fish.

FAQ

Q: How do you ensure the accuracy and reliability of the sequencing data?
A: We employ advanced bioinformatics tools and algorithms for data analysis, coupled with stringent quality control measures throughout the sequencing process to ensure high accuracy and reliability.
Q: What is the typical turnaround time for repertoire analysis services?
A: The typical turnaround time ranges from 10 to 12 weeks, depending on the complexity of the project and the volume of samples.
Q: What types of samples can be used for repertoire analysis?
A: We can analyze a variety of sample types, including peripheral blood mononuclear cells (PBMCs), bone marrow, lymph nodes, and tissue biopsies.
Q: What kind of output data and reports can I expect?
A: You will receive a comprehensive report including raw sequencing data, processed data files, and detailed analyses of clonotypes, diversity metrics, and graphical representations of the immune repertoire.
Q: How do you deal with the amplification biases introduced during library preparation?
A: We use unique molecular identifiers (UMIs) during library preparation to accurately quantify and correct amplification biases, ensuring a more accurate representation of the immune repertoire.

Whether you are involved in drug discovery, disease diagnosis, or immunological research, Creative Biolabs provides valuable insights and supports your scientific endeavors. For more information or to discuss your specific requirements, please contact us.

Reference

Fu, Xi, et al. "High-throughput sequencing of the expressed torafugu (Takifugu rubripes) antibody sequences distinguishes IgM and IgT repertoires and reveals evidence of convergent evolution." Frontiers in Immunology 9 (2018): 251.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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