Repertoire analysis utilizes high-throughput sequencing and bioinformatics to study the diversity and specificity of B cell receptors (BCR), T cell receptors (TCR), and antibodies. This analytical method provides detailed insights into how the immune system responds to pathogens and other foreign substances, helping to identify the best weapons against diseases.
Repertoire analysis not only aids scientists in developing new drugs but also enhances the accuracy of disease diagnosis, advancing medical technology. By combining phage display with repertoire analysis, Creative Biolabs can more efficiently discover and validate high-affinity and high-specificity candidate molecules, significantly promoting the progress of medical research and clinical applications.
Quantitative Immune Repertoire for Small Lymphocytes
Phage display platform is a powerful technology used to generate and screen large libraries of peptides or antibodies. It plays a crucial role in repertoire analysis by providing a diverse set of sequences for further study. The integration of the phage display platform with repertoire analysis provides a robust method for discovering and characterizing high-quality candidate molecules, thereby accelerating advancements in medical research and clinical applications.
Analysis of IgM and IgT Repertoires from Takifugu rubripes
Fig. 1 Overall IgM (A) and IgT (B) repertoires across the three torafugu.1
Scientists analyzed the antibody library of torafugu and obtained 1.2 billion expressed antibody sequences from three healthy adults. The findings revealed convergence and evolution of CDR-H3 sequences in IgM and IgT, and a bias towards specific VDJ combinations in individual fish. Different components of IgM and IgT were also observed, suggesting varied mechanisms for diversity creation in the naïve immune system of fish.
A: We employ advanced bioinformatics tools and algorithms for data analysis, coupled with stringent quality control measures throughout the sequencing process to ensure high accuracy and reliability.
A: The typical turnaround time ranges from 10 to 12 weeks, depending on the complexity of the project and the volume of samples.
A: We can analyze a variety of sample types, including peripheral blood mononuclear cells (PBMCs), bone marrow, lymph nodes, and tissue biopsies.
A: You will receive a comprehensive report including raw sequencing data, processed data files, and detailed analyses of clonotypes, diversity metrics, and graphical representations of the immune repertoire.
A: We use unique molecular identifiers (UMIs) during library preparation to accurately quantify and correct amplification biases, ensuring a more accurate representation of the immune repertoire.
Whether you are involved in drug discovery, disease diagnosis, or immunological research, Creative Biolabs provides valuable insights and supports your scientific endeavors. For more information or to discuss your specific requirements, please
.All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
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