Custom SARS-CoV-2 Virus-Like Particle (VLP) Production Service

Virus-Like Particles (VLPs) are made of viral capsid proteins that self-assemble into particulate structures closely resembling the natural virus from which they are derived. These VLPs are replication as well as infection incompetent, lacking regulatory proteins as well as infectious. In addition, VLPs possess several features including can be rapidly produced in large quantities through existing expression systems, highly resembling native viruses in terms of conformation and appearance, and displaying a repeated cluster of epitopes. Creative Biolabs has long time been the world-leading provider of VLPs. With the vast experience and advanced platforms, we are pleased to provide a complete line of VLPs production service based on our advanced mammalian cells system and insect system, also labeled with Green Fluorescent Protein (GFP), to help customers reach their project goals.

As mimicking natural virus structures, VLPs have evolved to become a widely accepted technology used for humans which are safe, highly efficacious and profitable. VLPs can consist of single or multiple proteins then assemble into the structure with single or multiple layers. Their capsids can be modified via genetic insertion or chemical conjugation which facilitating the multivalent display of a homologous or heterogeneous epitope antigen.

Figure.1 Non-enveloped or enveloped VLPs. (Ding, 2018)

In late December 2019, a cluster of pneumonia cases caused by a novel coronavirus (CoV) was reported in Wuhan, China. SARS-CoV-2 is the etiological agent responsible for the global COVID-19 outbreak.

Figure.2 Locations with Confirmed COVID-19 Cases as of July 16, 2020. (Source from CDC)

Structurally, SARS-CoV-2 has four main structural proteins including spike (S) glycoprotein, small envelope (E) glycoprotein, membrane (M) glycoprotein, and nucleocapsid (N) protein, and also several accessory proteins. Non-infectious VLPs displaying essential viral proteins can be used to study the structural properties of the SARS-CoV-2 virions and due to their maximum immunogenicity are also vaccine candidates. VLPs are released from cells with similar mechanisms as fully infectious virions and resemble the shape and composition of fully infectious virions. Currently, Creative Biolabs has successfully created VLPs composed of S, M, and E or S, M, N, E in mammalian cells and/or insect cell expression system (baculovirus).

Figure.3 Structure of SARS-CoV-2. (Kim, 2020)

Creative Biolabs offers one-step VLP production services using state-of-the-art virology techniques. The process details are always guided by the needs of our customers. The general process includes:

Figure.4 Production, purification and characterization of VLPs.

Creative Biolabs now offers high-quality VLP product services for customers in the world. Our seasoned scientific staff will work closely with you to keep you apprised of project progress while taking all your goals into account. Now, we are proud to provide SARS-CoV-2 VLP products, containing S/M/E or S/M/E/N to advance SARS-CoV-2 researches. What’s more, we provide VLP products labeled with GFP if customers want to put the dye marker for VLPs. Please feel free to search our catalog for any peptide products. If you cannot find the suitable products you desire, please do not hesitate to contact us.

References

Ding, X.; et al. Virus‐Like Particle Engineering: From Rational Design to Versatile Applications. Biotechnology journal. 2018, 13(5): 1700324.
Kim, D.; et al. The architecture of SARS-CoV-2 transcriptome. Cell. 2020.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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