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WB & IP-Positive Antibody Discovery Service

Overview Importance Service Categories Key Features

At Creative Biolabs, our custom hybridoma development services provide a strong guarantee for high-quality antibody production. Our hybridoma antibody generation process includes extensive assays to ensure antibodies have high specificity and affinity under unique assay conditions.

Overview of Western Blot (WB) & Immunoprecipitation (IP)

WB & IP are indispensable tools for antibody functional verification.

WB is a crucial strategy for determining antibody specificity. WB experiments are usually performed under protein denaturing conditions. Therefore, changes in the native structure of the antigen need to be considered during the development of antibodies for WB. Being able to recognize denatured antigens is an important step in verifying antibody function. A key indication that the desired antibody is specific for the target is the observation of a single band at a specific location.

Fig.1 Detection of spliceosome protein by WB. (Yamauchi & Herr, 2017)Fig.1 Spliceosome protein localization and expression in single mammalian cells.1

IP is a popular tool that allows the capture of target proteins from complex mixtures. Since it is very effective for enriching specific tags, IP has gradually become a powerful method when dealing with low-abundance proteins. In addition, IP plays an irreplaceable role in studying the interaction of target proteins with other molecules. Therefore, antibodies used for IP need to be validated in multiple assays to ensure efficient collection of the desired protein from a specific sample.

Why Perform WB & IP Verification

The incubating antibody in the IP experiment and the primary antibody in the WB experiment are crucial factors affecting the results of IP-WB. The former targets the bait protein in IP experiments, while the latter can target the bait protein or its interacting proteins.

Service Categories

To obtain hybridoma clones for IP and WB detection, we performed the IP or WB assays during the specific screening of sub-clones. Our WB&IP positive hybridoma screening ensures that we ultimately obtain specific hybridoma clones that recognize the target antigen and exhibit minimal cross-reactivity. Then the antibodies obtained by the hybridoma clones will be tested for IP or WB assays to ensure they have good reproducibility in WB & IP.

Why Choose Us?


As a leader in the production of hybridoma antibodies, we are willing to provide you with a full range of WB & IP hybridoma screening services. If you are looking for WB&IP-positive hybridoma development services, please feel free to contact us for more information.

Reference
  1. Yamauchi, Kevin A., and Amy E. Herr. "Subcellular western blotting of single cells." Microsystems & Nanoengineering 3.1 (2017): 1-9.
    Distributed under Open Access license CC BY 4.0, without modification.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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