With extensive experience and advanced technology, Creative Biolabs offers premier yeast display library screening services worldwide. Our excellent biopanning strategies and state-of-the-art equipment enable us to provide the best solutions. Our scientists tailor the most appropriate strategies to screen our customers' libraries effectively.
Yeast display is a cutting-edge technology used to present proteins, peptides, or antibody fragments on the surface of yeast cells. This powerful technique enables the high-throughput screening of large libraries to identify variants with optimal properties such as high binding affinity and specificity.
Yeast Display Library Screening
By coupling yeast antibody display libraries with fluorescence-activated cell sorting (FACS), high-affinity antibodies that bind specifically to targeted antigens can be selected using labeled target antigens. This combination provides a more controlled and quantitative selection process, enhancing the enrichment of clones with specific binding properties more effectively than phage display.
Creative Biolabs' yeast display screening services are designed to provide comprehensive and customized solutions for the discovery and characterization of high-affinity, specific binders to meet your specific research and development needs. Our services can significantly advance research, diagnostics, and therapeutics by leveraging advanced screening technologies and expert knowledge in yeast display systems.
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Yeast Display Antibody Library Screening Against Target Protein
Round 1 MACS Screening
MACS-based initial screening is conducted. We performed tests of scFv display and the target protein binding for output cells by FACS after screening. The staining signals of the display tag are good, and a positive enrichment can be observed for the target protein with the binding signal >20%. The enriched pools also had a strong binding signal to the control protein.
Round 2 FACS Sorting
FACS Sorting against the target protein is performed and competed with a control protein with sequence similarity of more than 90% to the target protein. The results showed a weak positive signal in the target protein group. The clones from R3 Gate G (5.30%) are randomly picked and performed binding tests and positive clones are continued with DNA sequencing.
our team of experts to find out more about our Yeast Display Services.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
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