Magic™ Custom Ion Channel Preparation Service

Creative Biolabs offers one-stop, custom-oriented ion channel production services based on our leading-edge Magic™ technique platform. Currently, we have developed, optimized, and commercialized multiple strategies for ion channel preparation for drug discovery, assay development, structural analysis, etc.

Overview of Ion Channels

Ion channels are pore-forming integral membrane proteins that constitute the passage of ions across plasma membranes. In the human genome, there are almost 400 different ion channels located on a broad range of cell types, governing various biophysical activities. According to ion conductivity, they can be generally classified into sodium, potassium, calcium, chloride, and non-selective cation channels. Ion channels play a critical role in regulating the electrical properties of excitable cells (e.g. neurons and myocytes), hormonal secretion, and blood pressure modulation. Their dysfunction is associated with diverse pathophysiology, including neuromuscular disorders, kidney diseases, pain, hypertension, etc. However, the discovery and development of new ion channel drug candidates remains an arduous task due to the complex and potentially species-specific physiology, difficulties in target identification, lack of strategies to discover and validate functional leads, and so on. With years of expertise in membrane protein studies and multiple state-of-the-art Magic™ membrane protein platforms, Creative Biolabs now provides professional service of ion channel production.

Strategies of Ion Channels Production

Ion channel expression systems

Creative Biolabs has rich experience in ion channel heterologous expression in cell-based E. coli, yeast, insect, and mammalian cell expression systems. Exhaustive condition optimization will be performed to improve the yield. We also provide cell-free expression systems, including E. coli lysate, wheat germ lysate, rabbit reticulocyte lysate, insect cell lysate, and mammalian cell lysate. Cell-free machinery relies on in vitro transcription/translation to generate difficult-to-express proteins, especially ion channels.

Ion channels solubilizers

With the help of Our detergent screening platform & lipid screening platform, we can reconstitute the resulting proteins in detergent micelles, liposomes, nanodiscs, and polymers without compromising native structure and functionalities. We also display the membrane portion in virus-like particles (VLPs). MP-VLPs technology can capture integral ion channels in their native conformation directly from the cell surface.

Ion channels stable cell lines

Creative Biolabs has established ion channel overexpression stable cell lines, which cover most of the currently hot targets, like Nav1.7. We can also construct a new stable strain upon custom request, with high expression levels of up to 5 million per cell. These well-characterized, ready-to-use cell lines make perfect tools for ion channel extraction, whole-cell immunization, and various cell-based assays.

Creative Biolabs is always striving for excellence in membrane protein-related studies and is determined to deliver the most satisfactory service/products to our worldwide clients. In addition to ion channels, we are also professional in tailoring impeccable service of GPCRs and transporters. Please contact us for more information.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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