Creative Biolabs currently presents to our worldwide clients multiple well-tailored premade single domain antibody (sdAb or domain antibody) libraries originated from camels and llamas. We are happy to leverage our unparalleled library resources and extensive expertise to assist our clients with their sdAb discovery and development projects.

Single domain antibodies, derived from camelid species, represent a special type of antibody consisting of a single monomeric variable antibody domain. With a molecular weight of only 12-15 kDa, single domain antibodies are much smaller than common antibodies (150-160 kDa). Therefore, single domain antibodies combine the benefits of conventional antibodies with important features of small molecule drugs, gaining increasing attention in biological drug development field in recent years.

Advantages of Single Domain Antibodies:

• Better solubility
• Able to bind to hidden epitopes that are not accessible to whole antibodies
• Greater affinity and selectivity, thus promising lower side effect and better efficacy
• Penetrate tissues faster than other antibodies, and even break through the blood-brain barrier
• Higher stability to changes in temperature and chemical environments, greater potential of gastrointestinal stability and oral availability
• Exceptional drug format flexibility and allow efficient drug discovery and development

Currently, there are following premade sdAb libraries in Creative Biolabs available for licensing:

CaV_HHL-1: Camel Naïve Single Domain Antibody Library

1. Phagemid phage display system
2. M13 pIII fusion containing 6×His and c-Myc detection tag
3. V_HH antibody genes from camel PBMCs
4. Capacity: 1.5×10⁹
5. Proliferation in TG1 cells
6. Production of soluble antibody fragments in HB2151
7. Helper phage M13KO7

LlaV_HHL-1: Llama Naïve Single Domain Antibody Library

1. Phagemid phage display system
2. M13 pIII fusion containing 6×His and c-Myc detection tag
3. V_HH antibody genes from llama lymphocytes
4. Capacity: 2.0×10⁹
5. Proliferation in TG1 cells
6. Production of soluble antibody fragments in HB2151
7. Helper phage M13KO7

Furthermore, we also provide Premade SdAb Library Screening service to identify specific binders for different kinds of targets from the above libraries and the following two libraries.

CaV_HHL-3: Camel Naïve Single Domain Antibody Library

1. Phagemid phage display system
2. M13 pIII fusion containing 6×His and c-Myc detection tag
3. V_HH antibody genes from camel PBMCs
4. Capacity: 3.0×10⁹
5. Proliferation in TG1 cells
6. Production of soluble antibody fragments in HB2151
7. Helper phage M13KO7

CaV_HHL-4: Camel Naïve Single Domain Antibody Library

1. Phagemid phage display system
2. M13 pIII fusion containing 6×His and c-Myc detection tag
3. V_HH antibody genes from camel lymphocytes
4. Capacity: 2.63×10¹⁰
5. Proliferation in TG1 cells
6. Production of soluble antibody fragments in HB2151
7. Helper phage M13KO7

CaV_HHL-5: Camel Naïve Single Domain Antibody Library

1. Phagemid phage display system
2. M13 pIII fusion containing HA and 6xHis detection tag
3. V_HH antibody genes from camel lymphocytes
4. Capacity: 3.0×10¹⁰
5. Proliferation in TG1 cells
6. Production of soluble antibody fragments in HB2151
7. Helper phage M13KO7

V_HHL-S1: Llama Synthetic Single Domain Antibody Library

1. Phagemid phage display system
2. M13 pIII fusion containing short HA and 6xHis detection tag
3. V_HH format
4. Introduced synthetic trimer-codon HCDR1, HCDR2 and HCDR3
5. Capacity: 1.0×10¹¹
6. Proliferation in TG1 cells
7. Production of soluble antibody fragments in HB2151
8. Helper phage M13KO7

V_HHL-S2: Camel Synthetic Single Domain Antibody Library

1. Phagemid phage display system
2. M13 pIII fusion containing short HA and 6xHis detection tag
3. V_HH format
4. Introduced synthetic trimer-codon HCDR1, HCDR2 and HCDR3
5. Capacity: 5.4×10¹⁰
6. Proliferation in TG1 cells
7. Production of soluble antibody fragments in HB2151
8. Helper phage M13KO7

V_HHL-S3: Camel Synthetic Single Domain Antibody Library

1. Phagemid phage display system
2. M13 pIII fusion containing 6xHis detection tag
3. V_HH format
4. Introduced synthetic trimer-codon HCDR3
5. Capacity: 2.2×10¹⁰
6. Proliferation in TG1 cells
7. Production of soluble antibody fragments in HB2151
8. Helper phage M13KO7

V_HHL-S4: Camel Semi-synthetic Single Domain Antibody Library

1. Phagemid phage display system
2. M13 pIII fusion containing short HA and 6xHis detection tag
3. V_HH format
4. Introduced synthetic trimer-codon HCDR3 in naïve V_HH gene pool (lacking HCDR3)
5. Capacity: 5.3×10¹⁰
6. Proliferation in TG1 cells
7. Production of soluble antibody fragments in HB2151
8. Helper phage M13KO7

In addition, we are also happy to tailor custom-oriented services of sdAb library construction with different of strategies, such as NNK/NNS, trimer codon, and then perform library screening against a variety of targets from them. Our tech staffs are ready 24/7 to discuss with your every specific project. Please feel free to inquire us for more information.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.