Yeast Cell based Virus-Like Particle (VLP) Production Service

Creative Biolabs has been long-term committed to developing virus-like particle (VLP) technology in yeast cell systems. We are honored to use our advanced platforms and extensive experience to serve our valued customers with the best service and the most qualified products.

Introduction of VLP Production in Yeast Cell Expression System

As a eukaryotic organism, yeast has become an extremely appealing expression system for the production of VLPs and heterologous proteins. As yeast strains employed in the laboratory are nonpathogenic and harmless organisms, they are safe and can be manipulated without any extra care. Compared to mammalian-based systems, they have a number of benefits, such as well-established fermentative growth, cheap and readily available medium requirements, quick growth rates, and ease of genetic manipulation. Yeast-based systems are appropriate for large-scale production because of these qualities. In addition, yeast expression systems facilitate the synthesis of recombinant glycoproteins and one or more viral capsid proteins that form and show a wide range of antigenic sites, including discontinuous epitopes.

VLPs can be produced in different expression systems. (Srivastava, et al. 2023) Fig.1 Saccharomyces cerevisiae and Komagataella phaffii remain the most commonly used yeast species.^1,3

Advantages of VLP Production in Yeast Expression System

Higher yield: comparative studies between yeast and E. coli systems suggested approximately 30% higher yield of Qb VLPs expressed in Pichia than that is obtained in E. coli.
Soluble expression: yeast host can generate soluble VLP products while for E. coli, in most cases, the heterologous products tend to form inclusion bodies.
Free of endotoxin, which can be a severe problem in vaccine development.
Post-translational modifications: several post-translational modiﬁcations such as glycosylation or phosphorylation can be possibly introduced to VLPs.
Suitable for non-enveloped VLP production.

Comparison of VLP Production in the Different Yeast Species

The majority of the time, VLPs made in various yeast species can contain both distinct and identical viral proteins. The purification procedure for HEV VLPs, for example, was simpler than that of other systems since the identical viral protein was generated in all three yeast species except for P. pastoris, where the VLPs were secreted. Furthermore, S. cerevisiae and P. pastoris have been effectively used to create VLPs made of various viral proteins. Creative Biolabs has already proven that several VLPs can be successfully obtained using yeast cells. We now provide multiple yeast strains as powerful expression machinery for VLP production, including Pichia pastoris, Hansenula polymorpha, saccharomyces cerevisiae, etc.

VLPs production in the yeast expression system. (Brachelente, et al. 2023) Fig.2 Comparison of three yeast species.^2,3

Creative Biolabs now offers unparalleled virus-like particle (VLPs) service based on yeast cell systems. We are able to provide a broad range of VLPs-related services from production, optimization, and characterization to functionalization strategies. Please feel free to contact us for further discussions.

References

Srivastava, Vartika, et al. "Yeast-based virus-like particles as an emerging platform for vaccine development and delivery." Vaccines 11.2 (2023): 479.
Brachelente, Sara, Alvaro Galli, and Tiziana Cervelli. "Yeast and Virus-like Particles: A Perfect or Imperfect Couple?" Applied Microbiology 3.3 (2023): 805-825.
Distributed under Open Access license CC BY 4.0, without modification.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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