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Yeast Display Library Screening Services

With extensive experience and advanced technology, Creative Biolabs offers premier yeast display library screening services worldwide. Our excellent biopanning strategies and state-of-the-art equipment enable us to provide the best solutions. Our scientists tailor the most appropriate strategies to screen our customers' libraries effectively.

Background Services Advantages Applications Case

What is Yeast Display?

Yeast display is a cutting-edge technology used to present proteins, peptides, or antibody fragments on the surface of yeast cells. This powerful technique enables the high-throughput screening of large libraries to identify variants with optimal properties such as high binding affinity and specificity.

Yeast Display Library Screening

By coupling yeast antibody display libraries with fluorescence-activated cell sorting (FACS), high-affinity antibodies that bind specifically to targeted antigens can be selected using labeled target antigens. This combination provides a more controlled and quantitative selection process, enhancing the enrichment of clones with specific binding properties more effectively than phage display.

Yeast Display Library Screening Services - Rapid Identification of High-Affinity Binders

Creative Biolabs' yeast display screening services are designed to provide comprehensive and customized solutions for the discovery and characterization of high-affinity, specific binders to meet your specific research and development needs. Our services can significantly advance research, diagnostics, and therapeutics by leveraging advanced screening technologies and expert knowledge in yeast display systems.

Workflow

Fig. 1 Workflow of yeast display library screening. (Creative Biolabs Original)


Related Services Available

Advantages of Yeast Display Library Screening for Binder Discovery


Yeast Display Platform
Continually improving our yeast display platform ensuring proper folding and post-translational modifications of displayed proteins to provide cutting-edge solutions.

Wide Range of Targets
Screen against various targets, including small molecules, proteins, peptides, and cells.

High-throughput and Quantitative Screening
High-throughput techniques for rapidly screening millions of yeast cells, facilitate the efficient identification of high-affinity binders.

Rapid Screening Cycles
Relatively fast screening and enriching high-affinity binders, allowing for the rapid identification and characterization of potential binders.

Characterization
Characterize binders through yeast surface titrations and DNA sequencing, providing detailed insights into binding affinity and specificity.

Integration with Other Methods
Combination with other selection methods, such as MACS and phage display, to further enhance the selection process and identify the best candidates for binder development.

Applications

  • Antibody discovery.
  • Affinity maturation.
  • Humanization.
  • Targeting membrane proteins.
  • Screening against challenging targets.
  • Creating novel constructs such as ADCs and bispecific antibodies.
  • Identify enzyme inhibitor.
  • Map epitopes on antigens.
  • Discover/Validate new therapeutic targets.
  • Discover interaction between ligand and receptor.

Cases

Yeast Display Antibody Library Screening Against Target Protein

Round 1 MACS Screening

Fig. 1 Workflow of yeast display library screening. (Creative Biolabs Original)

MACS-based initial screening is conducted. We performed tests of scFv display and the target protein binding for output cells by FACS after screening. The staining signals of the display tag are good, and a positive enrichment can be observed for the target protein with the binding signal >20%. The enriched pools also had a strong binding signal to the control protein.

Round 2 FACS Sorting

Fig. 2 Round one of yeast display antibody library screening. (Creative Biolabs Original)

FACS Sorting against the target protein is performed and competed with a control protein with sequence similarity of more than 90% to the target protein. The results showed a weak positive signal in the target protein group. The clones from R3 Gate G (5.30%) are randomly picked and performed binding tests and positive clones are continued with DNA sequencing.

Contact our team of experts to find out more about our Yeast Display Services.


All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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