Yeast Display based Binder Discovery Services

Yeast display, also known as yeast surface display, is an innovative technique extensively used to express proteins on the surface of yeast cells. This method leverages the eukaryotic system of yeast, which ensures proper protein folding, modification, and translocation before surface display. Capitalizing on these benefits, Creative Biolabs has developed the Ultraff™ yeast display platform, a unique and advanced system for constructing yeast display libraries, screening, and antibody affinity maturation, among other applications.

Services Platform Advantages Cases

Yeast Display based Binder Discovery

With extensive experience accumulated over decades, Creative Biolabs has established the unique Ultraff™ Yeast Display System, a highly robust platform that utilizes the expression of recombinant proteins on the surface of yeast cells to isolate and engineer high-affinity binders, such as antibodies or peptides. This advanced system allows us to selectively isolate yeast cells displaying binders with desired affinities and specificities, ensuring the highest quality outcomes. We have successfully discovered and optimized monoclonal antibodies with picomolar (pM) affinity, the highest ever achieved through in vitro recombinant antibody production technology. We collaborate closely with you to understand your specific needs and tailor our services and products to meet your goals effectively.

Comprehensive Services

Creative Biolabs offers a robust yeast display system designed to optimize the discovery and development of proteins or antibodies. At Creative Biolabs, we offer a suite of services tailored to support your yeast display-based binder discovery projects:

Custom Yeast Display Library Construction

Diverse genetic materials.
Custom library design.
Efficient transformation protocol.
High diversity libraries.

Yeast Display Library Screening

FACS-based screening.
Selective isolation high-affinity binders.

Yeast Display-Based Antibody Affinity Maturation

Enhancement of the affinity and specificity.
Engineering and optimizing with improved performance.

Yeast Display-Based T Cell Receptor Engineering

Specialized T cell receptors for various therapeutic applications.
Custom Solutions to unique project requirements.

Human Monoclonal Antibody Identification Through Phage & Yeast Display Combination

Dual display platforms to maximize antibody potential.
Synergistic Approach to isolate antibodies with high purity and yield.

Yeast Display-Based Protein Optimization and Engineering

Protein Enhancement
Tailored Solutions

Yeast Display-Based Antibody Discovery Service

Robust Discovery Platform
Comprehensive Screening

Yeast Display-Based Peptide Discovery

Diverse Peptide Libraries
High-Throughput Identification

Ultraff™ Yeast Display Platform

Yeast surface display, often referred to as yeast display, is a powerful tool for protein engineering with diverse applications in biotechnology and biomedicine. It is widely utilized for in vitro protein evolution. This technique serves as an alternative for isolating and engineering antibody fragments, such as Fab, scFv, and sdAb, from both immune and non-immune libraries. Yeast display has been successfully applied to isolate recombinant antibodies with binding specificity to various proteins, peptides, small molecules, and T cell receptors.

Key Features of Ultraff™ Yeast Display

Genotype-Phenotype Link
Much like phage display, yeast display establishes a direct connection between genotype and phenotype. The gene of interest is housed within a plasmid inside the yeast cells, and the encoded antibody is expressed on the cell surface.
High Display Level
Each yeast cell displays 10,000 to 100,000 copies of the protein of interest.
Quantitative Screening
Fluorescence-activated cell sorting (FACS) is used to quantify surface expression and avidity, measuring both antigen binding strength and antibody expression levels.
Precise Selection
FACS enables precise control of selection parameters, including binding population percentage, signal normalization, and desired binding affinities, through flow cytometry.
Enrichment Rounds
Typically, 3-5 rounds of enrichment are conducted to isolate target antigen-binding clones.
Specificity and Reproducibility Validation
Antibodies displayed by single yeast colonies are evaluated for specificity and reproducibility. If functional, the antibody gene is sequenced for validation.

Applications of Ultraff™ Yeast Display

Therapeutic Antibody Discovery

Monoclonal Antibody Discovery

Antibody Affinity Maturation

Antibody Humanization

Protein Interaction Studies

Benefits of Choosing Creative Biolabs

Expertise

Our team brings decades of experience in yeast display technology and binder discovery, offering unparalleled knowledge and skills.

Innovation

We use cutting-edge techniques and proprietary platforms, ensuring the highest quality and efficiency in our services.

Customization

We offer tailored solutions to meet your specific project needs, providing both flexibility and precision.

Comprehensive Support

From initial consultation to final delivery, our dedicated team provides complete support, ensuring your project's success.

Cases

Yeast Surface Display and Antigen Binding Test

Fig. 2 A FC result of yeast surface display and antigen binding test. (Creative Biolabs Original)

Results

The positive staining results of anti-myc/HA tag antibodies revealed that the display of scFv antibody on yeast cell surface is successful.
The positive binding results of biotin-Ag revealed that the scFv antibody is displayed in the correct conformation on the yeast cell surface.

Harness the power of our yeast display services to elevate your protein engineering projects. Contact us today to discuss your specific needs and discover how our customized solutions can advance your research or biotech initiatives.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

	USA: Europe: Germany:
	Call us at: USA: UK: Germany:
	Fax:
	Email: