Genome Editing Screening Service for Infectious Disease Research

Genome editing screening can precisely modify the genome of cells or organisms to simulate the infection process and anti-infective effects, thus researching and screening for anti-infective hits. Creative Biolabs provides efficient genome editing screening services and innovative research models to assist clients in anti-infect hit discovery.

Genome Editing Screening Types

• Gene Knockout Screening
  Gene knockout screening is the removal of specific genes from the genome through gene editing technology, which in turn screens and identifies key genes associated with the infection process and understands their role in infection.
• Gene Knockdown Screening
  Knockdown screening is a method of screening for promoter genes or causative agents associated with infectious diseases by reducing the expression level of the target gene to inhibit its function. This is usually achieved with shRNA or siRNA.
• Gene Activation Screening
  Gene activation screening typically involves the use of gene regulatory elements (e.g., promoters) or transcription factors to increase the expression level of the target gene to enhance its function. Gene activation screening allows researchers to identify protective or immune response genes associated with the infection process and explore their protective role in infection.
• Gene Overexpression Screening
  Gene overexpression screens are performed by introducing target genes into cells and causing them to be overexpressed, usually through using viral vectors or plasmids, in order to explore the role of facilitator genes or disease-causing factors associated with the infection process in the promotion of infection.
• Customized Lentiviral Screening
  Customized Lentiviral Screening efficiently introduces genetic material into dividing and non-dividing cells utilizing customized lentiviral vectors, allowing for the rapid and efficient identification of genes associated with the infection process among a large number of target genes, while providing reproducibility and high sensitivity.
• Deconvolution
  Deconvolution allows for the isolation of individual components from complex mixtures in infectious disease studies to analyze complex biological samples such as infected tissues or body fluids in infection models. Working with algorithms and statistical models, researchers can identify and quantify different cell types, viral particles, and other biological components of the infection process to understand the interactions between the different components of the infection process and the mechanism of action of drug targets.

Fig. 1 Co-correlation analysis screens for genes of interest that are in the same cell survival pathway.¹

Genome Editing Screening Service Process

Advantages of Genome Editing Screening Service

• Our team of scientists has accumulated rich experience in the field of genome editing and anti-infective hit research.
• Equipped with the most advanced gene editing technology and equipment, we are able to perform genome-wide target screening and validation to ensure experimental accuracy and efficiency.
• According to your specific requirements and research objectives, we provide targeted genome editing screening experimental design and solutions.
• Strictly follow international standards and norms for quality control of gene editing screening to ensure the reliability of the experimental process and results.

Creative Biolabs provides genome editing screening services aimed at screening potential drug targets associated with infectious diseases on a genome-wide scale. If you are interested in our genome editing screening service or have any questions, please feel free to contact us.

Reference:

1. Przybyla, Laralynne, and Luke A. Gilbert. "A new era in functional genomics screens." Nature Reviews Genetics 23.2 (2022): 89-103.

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• Antibody/Peptide Discovery
• Ebola Virus