Tandem scFv Generation Service

Based on years of experience in antibody discovery and production, Creative Biolabs has developed second to none bispecific antibody (BsAbs) generation services. With our comprehensive platform, we are proud to offer expected tandem scFv with high affinity and low immunogenicity for both academic and clinical purposes.

Just as its name implies, two moieties of scFv string together to form tandem scFv, which has 1+1 antigen-binding. Tandem scFv can be expressed by transfected mammalian cells with four tandem variable regions connected by flexible peptides. Without Fc region, its smaller size (~55 kDa) gives it a short serum half-life, meanwhile, it also ensures a closer proximity to the two targets or the surface of two target cells. Based on tandem scFv, more than moieties of scFv can be stringed to form multi-specific or bispecific, multivalent BsAbs. And CH3 of Fc regions are also engineered into tandem scFv to expand its half life of Fc effector functions, resulting in tandem scFv-CH3 or tandem scFv-Fc.

Figure 1. The constructs of Tandem scFv, Tandem scFv-CH3, Tandem scFv-Fc

Tandem scFv in Immunnology

Due to cancer cells subverting mechanisms of T-cell control under selective pressure, the antitumor response of T-cells is usually insufficient in part. Improved understanding of the mechanisms of evasion of immune surveillance has result in a number of novel methods of T-cell based immunotherapy. Among of which, BsAbs are a potential strategy. Tandem scFv has more than thousands of times of efficiency in lysing tumor cell compared with other CD3-bispecific or IgG antibodies. All of these properties have made it one of the most important T cell recruiters for cancer treatment and immune regulation.

With the well-established antibody generation platforms, the experienced scientists here at Creative Biolabs are dedicated to helping you develop unique tandem scFv and also providing custom Tandem scFv products linking two or more scFvs against hot targets. We also provide other various services regarding BsAbs development. Please feel free to contact us for more information and a detailed quote.